- 蛋白/抗體共 218258 條
FLJ35834 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
FLJ35834蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC091520,CD171721 [Species物種來源]: Homo sapiens 【 FLJ35834蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FLJ35834 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FLJ35834 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FLJ35834 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FLJ35834 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens FLJ35834 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FLJ35834 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FLJ35834 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
DDX10 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
DDX10蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC091521,CD359376 [Species物種來源]: Homo sapiens 【 DDX10蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DDX10 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DDX10 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DDX10 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DDX10 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens DDX10 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DDX10 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DDX10 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FLJ23436 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
FLJ23436蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092403,BQ939342 [Species物種來源]: Homo sapiens 【 FLJ23436蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FLJ23436 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FLJ23436 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FLJ23436 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FLJ23436 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens FLJ23436 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FLJ23436 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FLJ23436 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092404,BU151157 [Species物種來源]: Homo sapiens 【 EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 EPAC,EPAC1,BCM910,RAPGEF3,MGC21410,CAMP-GEFI Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KIF3C Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
KIF3C蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092406,BU509719 [Species物種來源]: Homo sapiens 【 KIF3C蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KIF3C Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KIF3C Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KIF3C protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KIF3C Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens KIF3C [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KIF3C Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KIF3C Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
UBCH9,UBCM2,UBE2E3 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
UBCH9,UBCM2,UBE2E3蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092407,BU151011 [Species物種來源]: Homo sapiens 【 UBCH9,UBCM2,UBE2E3蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ UBCH9,UBCM2,UBE2E3 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 UBCH9,UBCM2,UBE2E3 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens UBCH9,UBCM2,UBE2E3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 UBCH9,UBCM2,UBE2E3 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens UBCH9,UBCM2,UBE2E3 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 UBCH9,UBCM2,UBE2E3 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 UBCH9,UBCM2,UBE2E3 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CUL3 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CUL3蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092409,BU189717 [Species物種來源]: Homo sapiens 【 CUL3蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CUL3 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CUL3 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CUL3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CUL3 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens CUL3 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CUL3 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CUL3 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092411,CD300497 [Species物種來源]: Homo sapiens 【 RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RGS22,FLJ40080,PRTD-NY2,MGC102908,DKFZP434I092 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092412,CD251830 [Species物種來源]: Homo sapiens 【 SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SGOL2,KCTD18,TRIPIN,FLJ25211,MGC129665 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
LOC550631 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
LOC550631蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092419,CD251966 [Species物種來源]: Homo sapiens 【 LOC550631蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ LOC550631 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 LOC550631 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens LOC550631 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 LOC550631 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens LOC550631 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 LOC550631 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 LOC550631 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
C12ORF29 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C12ORF29蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092422,CD360066 [Species物種來源]: Homo sapiens 【 C12ORF29蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C12ORF29 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C12ORF29 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C12ORF29 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C12ORF29 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens C12ORF29 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C12ORF29 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C12ORF29 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF533 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZNF533蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092423,CD244390 [Species物種來源]: Homo sapiens 【 ZNF533蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF533 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF533 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF533 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF533 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens ZNF533 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF533 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF533 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
THAP3 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
THAP3蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092427,CD245750 [Species物種來源]: Homo sapiens 【 THAP3蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ THAP3 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 THAP3 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens THAP3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 THAP3 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens THAP3 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 THAP3 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 THAP3 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SNAT,AANAT,AA-NAT Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SNAT,AANAT,AA-NAT蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092430,CD243881 [Species物種來源]: Homo sapiens 【 SNAT,AANAT,AA-NAT蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SNAT,AANAT,AA-NAT Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SNAT,AANAT,AA-NAT Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SNAT,AANAT,AA-NAT protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SNAT,AANAT,AA-NAT Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens SNAT,AANAT,AA-NAT [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SNAT,AANAT,AA-NAT Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SNAT,AANAT,AA-NAT Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
AYTL3,AGPAT7,FLJ10257,LPAAT-ETA蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092463,CF993928 [Species物種來源]: Homo sapiens 【 AYTL3,AGPAT7,FLJ10257,LPAAT-ETA蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AYTL3,AGPAT7,FLJ10257,LPAAT-ETA protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens AYTL3,AGPAT7,FLJ10257,LPAAT-ETA [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AYTL3,AGPAT7,FLJ10257,LPAAT-ETA Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CALC2,CALCB,CGRP2,CGRP-II,FLJ30166蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092468,BI668461 [Species物種來源]: Homo sapiens 【 CALC2,CALCB,CGRP2,CGRP-II,FLJ30166蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CALC2,CALCB,CGRP2,CGRP-II,FLJ30166 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
EBPL,EBRP Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
EBPL,EBRP蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092471,BG714162 [Species物種來源]: Homo sapiens 【 EBPL,EBRP蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ EBPL,EBRP Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 EBPL,EBRP Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens EBPL,EBRP protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 EBPL,EBRP Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens EBPL,EBRP [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 EBPL,EBRP Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 EBPL,EBRP Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GPR172B Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
GPR172B蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092473,CB959884 [Species物種來源]: Homo sapiens 【 GPR172B蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GPR172B Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GPR172B Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GPR172B protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GPR172B Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens GPR172B [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GPR172B Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GPR172B Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
LOC51334,MGC104614 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
LOC51334,MGC104614蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092474,CF593783 [Species物種來源]: Homo sapiens 【 LOC51334,MGC104614蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ LOC51334,MGC104614 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 LOC51334,MGC104614 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens LOC51334,MGC104614 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 LOC51334,MGC104614 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens LOC51334,MGC104614 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 LOC51334,MGC104614 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 LOC51334,MGC104614 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
RAB21,KIAA0118 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
RAB21,KIAA0118蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC092475,CD110134 [Species物種來源]: Homo sapiens 【 RAB21,KIAA0118蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RAB21,KIAA0118 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RAB21,KIAA0118 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RAB21,KIAA0118 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RAB21,KIAA0118 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens RAB21,KIAA0118 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RAB21,KIAA0118 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RAB21,KIAA0118 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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