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C6ORF120 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C6ORF120蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051700,BG714304 [Species物種來源]: Homo sapiens 【 C6ORF120蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C6ORF120 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C6ORF120 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C6ORF120 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C6ORF120 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens C6ORF120 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C6ORF120 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C6ORF120 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CPD Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CPD蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051702,BC051702.1,BI560221,BI560221.1 [Species物種來源]: Homo sapiens 【 CPD蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CPD Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CPD Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CPD protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CPD Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CPD [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CPD Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CPD Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MGC39606 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MGC39606蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051704,BC051704.1,BI547903,BI547903.1 [Species物種來源]: Homo sapiens 【 MGC39606蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MGC39606 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MGC39606 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MGC39606 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MGC39606 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens MGC39606 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MGC39606 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MGC39606 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051715,BC051715.1,BI545463,BI545463.1 [Species物種來源]: Homo sapiens 【 HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HNL3,NLGN3,ASPGX1,AUTSX1,KIAA1480 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
RIPX,RUFY3,KIAA0871 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
RIPX,RUFY3,KIAA0871蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051716,BC051716.1,BI549640,BI549640.1 [Species物種來源]: Homo sapiens 【 RIPX,RUFY3,KIAA0871蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RIPX,RUFY3,KIAA0871 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RIPX,RUFY3,KIAA0871 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RIPX,RUFY3,KIAA0871 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RIPX,RUFY3,KIAA0871 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens RIPX,RUFY3,KIAA0871 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RIPX,RUFY3,KIAA0871 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RIPX,RUFY3,KIAA0871 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
COD2,COG6,KIAA1134,DKFZP313D191蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051723,BC051723.1,BI547815,BI547815.1 [Species物種來源]: Homo sapiens 【 COD2,COG6,KIAA1134,DKFZP313D191蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ COD2,COG6,KIAA1134,DKFZP313D191 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 COD2,COG6,KIAA1134,DKFZP313D191 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens COD2,COG6,KIAA1134,DKFZP313D191 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 COD2,COG6,KIAA1134,DKFZP313D191 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens COD2,COG6,KIAA1134,DKFZP313D191 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 COD2,COG6,KIAA1134,DKFZP313D191 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 COD2,COG6,KIAA1134,DKFZP313D191 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SUHW3,ZNF633,FLJ20095 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SUHW3,ZNF633,FLJ20095蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051728,BC051728.1,BG718445,BG718445.1 [Species物種來源]: Homo sapiens 【 SUHW3,ZNF633,FLJ20095蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SUHW3,ZNF633,FLJ20095 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SUHW3,ZNF633,FLJ20095 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SUHW3,ZNF633,FLJ20095 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SUHW3,ZNF633,FLJ20095 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens SUHW3,ZNF633,FLJ20095 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SUHW3,ZNF633,FLJ20095 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SUHW3,ZNF633,FLJ20095 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
AOF2,LSD1,LUZP1,BHC110,KIAA0601蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051733,BC051733.1,BG772071,BG772071.1 [Species物種來源]: Homo sapiens 【 AOF2,LSD1,LUZP1,BHC110,KIAA0601蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AOF2,LSD1,LUZP1,BHC110,KIAA0601 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AOF2,LSD1,LUZP1,BHC110,KIAA0601 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AOF2,LSD1,LUZP1,BHC110,KIAA0601 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AOF2,LSD1,LUZP1,BHC110,KIAA0601 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens AOF2,LSD1,LUZP1,BHC110,KIAA0601 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AOF2,LSD1,LUZP1,BHC110,KIAA0601 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AOF2,LSD1,LUZP1,BHC110,KIAA0601 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PZF,CNTF,ZFP91,FKSG11 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
PZF,CNTF,ZFP91,FKSG11蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051743,BC051743.1,BG721319,BG721319.1 [Species物種來源]: Homo sapiens 【 PZF,CNTF,ZFP91,FKSG11蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PZF,CNTF,ZFP91,FKSG11 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PZF,CNTF,ZFP91,FKSG11 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PZF,CNTF,ZFP91,FKSG11 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PZF,CNTF,ZFP91,FKSG11 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PZF,CNTF,ZFP91,FKSG11 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PZF,CNTF,ZFP91,FKSG11 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PZF,CNTF,ZFP91,FKSG11 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TOP3A Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
TOP3A蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051748,BC051748.1,BQ226006,BQ226006.1 [Species物種來源]: Homo sapiens 【 TOP3A蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TOP3A Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TOP3A Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TOP3A protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TOP3A Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens TOP3A [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TOP3A Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TOP3A Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PI4K2B,PIK42B,PI4KIIB,FLJ11105蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051749,BC051749.1,BQ233049,BQ233049.1 [Species物種來源]: Homo sapiens 【 PI4K2B,PIK42B,PI4KIIB,FLJ11105蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PI4K2B,PIK42B,PI4KIIB,FLJ11105 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PI4K2B,PIK42B,PI4KIIB,FLJ11105 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PI4K2B,PIK42B,PI4KIIB,FLJ11105 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PI4K2B,PIK42B,PI4KIIB,FLJ11105 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PI4K2B,PIK42B,PI4KIIB,FLJ11105 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PI4K2B,PIK42B,PI4KIIB,FLJ11105 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PI4K2B,PIK42B,PI4KIIB,FLJ11105 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ABCF3,FLJ11198,EST201864 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ABCF3,FLJ11198,EST201864蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051754,BC051754.2,BQ214146,BQ214146.1 [Species物種來源]: Homo sapiens 【 ABCF3,FLJ11198,EST201864蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ABCF3,FLJ11198,EST201864 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ABCF3,FLJ11198,EST201864 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ABCF3,FLJ11198,EST201864 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ABCF3,FLJ11198,EST201864 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ABCF3,FLJ11198,EST201864 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ABCF3,FLJ11198,EST201864 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ABCF3,FLJ11198,EST201864 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CECR1,IDGFL Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CECR1,IDGFL蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051755,BC051755.1,BM921623,BM921623.1 [Species物種來源]: Homo sapiens 【 CECR1,IDGFL蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CECR1,IDGFL Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CECR1,IDGFL Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CECR1,IDGFL protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CECR1,IDGFL Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CECR1,IDGFL [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CECR1,IDGFL Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CECR1,IDGFL Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
BRT,BYK,DTK,RSE,SKY,TIF,TYRO3蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051756,BC051756.1,BQ212044,BQ212044.1 [Species物種來源]: Homo sapiens 【 BRT,BYK,DTK,RSE,SKY,TIF,TYRO3蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 BRT,BYK,DTK,RSE,SKY,TIF,TYRO3 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
C20ORF96,FLJ40075,DJ1103G7.2 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C20ORF96,FLJ40075,DJ1103G7.2蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051762,BC051762.1,BQ072883,BQ072883.1 [Species物種來源]: Homo sapiens 【 C20ORF96,FLJ40075,DJ1103G7.2蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C20ORF96,FLJ40075,DJ1103G7.2 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C20ORF96,FLJ40075,DJ1103G7.2 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C20ORF96,FLJ40075,DJ1103G7.2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C20ORF96,FLJ40075,DJ1103G7.2 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens C20ORF96,FLJ40075,DJ1103G7.2 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C20ORF96,FLJ40075,DJ1103G7.2 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C20ORF96,FLJ40075,DJ1103G7.2 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051764,BC051764.2,BQ223080,BQ223080.1 [Species物種來源]: Homo sapiens 【 XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 XPE,DDB1,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
NTE Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
NTE蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051768,BC051768.1,BM926350,BM926350.1 [Species物種來源]: Homo sapiens 【 NTE蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NTE Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NTE Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NTE protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NTE Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens NTE [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NTE Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NTE Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ACTN2 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ACTN2蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051770,BC051770.1,BQ948989,BQ948989.1 [Species物種來源]: Homo sapiens 【 ACTN2蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ACTN2 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ACTN2 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ACTN2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ACTN2 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ACTN2 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ACTN2 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ACTN2 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FLJ30707 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
FLJ30707蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051771,BC051771.1,BM546404,BM546404.1 [Species物種來源]: Homo sapiens 【 FLJ30707蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FLJ30707 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FLJ30707 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FLJ30707 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FLJ30707 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FLJ30707 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FLJ30707 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FLJ30707 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CBX7 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CBX7蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC051773,BC051773.1,BQ722296,BQ722296.1 [Species物種來源]: Homo sapiens 【 CBX7蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CBX7 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CBX7 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CBX7 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CBX7 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CBX7 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CBX7 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CBX7 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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