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CMTM3,CKLFSF3,FLJ31762,MGC51956 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CMTM3,CKLFSF3,FLJ31762,MGC51956蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047701,BC047701.1,BM928298,BM928298.1 [Species物種來源]: Homo sapiens 【 CMTM3,CKLFSF3,FLJ31762,MGC51956蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CMTM3,CKLFSF3,FLJ31762,MGC51956 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CMTM3,CKLFSF3,FLJ31762,MGC51956 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CMTM3,CKLFSF3,FLJ31762,MGC51956 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CMTM3,CKLFSF3,FLJ31762,MGC51956 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens CMTM3,CKLFSF3,FLJ31762,MGC51956 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CMTM3,CKLFSF3,FLJ31762,MGC51956 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CMTM3,CKLFSF3,FLJ31762,MGC51956 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF169,MGC51961 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZNF169,MGC51961蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047702,BC047702.2,BM457588,BM457588.1 [Species物種來源]: Homo sapiens 【 ZNF169,MGC51961蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF169,MGC51961 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF169,MGC51961 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF169,MGC51961 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF169,MGC51961 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens ZNF169,MGC51961 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF169,MGC51961 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF169,MGC51961 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PLA1A,PSPLA1,PS-PLA1 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
PLA1A,PSPLA1,PS-PLA1蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047703,BC047703.2,BQ431116,BQ431116.1 [Species物種來源]: Homo sapiens 【 PLA1A,PSPLA1,PS-PLA1蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PLA1A,PSPLA1,PS-PLA1 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PLA1A,PSPLA1,PS-PLA1 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PLA1A,PSPLA1,PS-PLA1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PLA1A,PSPLA1,PS-PLA1 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens PLA1A,PSPLA1,PS-PLA1 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PLA1A,PSPLA1,PS-PLA1 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PLA1A,PSPLA1,PS-PLA1 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SPATA3,TSARG1 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SPATA3,TSARG1蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047704,BC047704.1,BM908011,BM908011.1 [Species物種來源]: Homo sapiens 【 SPATA3,TSARG1蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SPATA3,TSARG1 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SPATA3,TSARG1 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SPATA3,TSARG1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SPATA3,TSARG1 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens SPATA3,TSARG1 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SPATA3,TSARG1 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SPATA3,TSARG1 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047707,BC047707.1,BM907329,BM907329.1 [Species物種來源]: Homo sapiens 【 NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NPN3,SRX1,SRXN1,YKL086W,C20ORF139,DJ850E9.2 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CYBASC3,MGC20446 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CYBASC3,MGC20446蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047710,BC047710.1,BQ430448,BQ430448.1 [Species物種來源]: Homo sapiens 【 CYBASC3,MGC20446蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CYBASC3,MGC20446 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CYBASC3,MGC20446 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CYBASC3,MGC20446 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CYBASC3,MGC20446 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens CYBASC3,MGC20446 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CYBASC3,MGC20446 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CYBASC3,MGC20446 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047711,BC047711.1,BQ226150,BQ226150.1 [Species物種來源]: Homo sapiens 【 S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 S19BP,FLJ21770,MGC52010,RPS19BP1,DJ1104E15.4 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CCDC95,FLJ00079,FLJ90652 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CCDC95,FLJ00079,FLJ90652蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047712,BC047712.2,BQ426035,BQ426035.1 [Species物種來源]: Homo sapiens 【 CCDC95,FLJ00079,FLJ90652蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CCDC95,FLJ00079,FLJ90652 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CCDC95,FLJ00079,FLJ90652 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CCDC95,FLJ00079,FLJ90652 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CCDC95,FLJ00079,FLJ90652 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens CCDC95,FLJ00079,FLJ90652 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CCDC95,FLJ00079,FLJ90652 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CCDC95,FLJ00079,FLJ90652 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SUZ12P Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SUZ12P蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047718,BC047718.1,BM562953,BM562953.1 [Species物種來源]: Homo sapiens 【 SUZ12P蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SUZ12P Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SUZ12P Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SUZ12P protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SUZ12P Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens SUZ12P [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SUZ12P Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SUZ12P Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TMC7,FLJ21240,DKFZP781O2274 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
TMC7,FLJ21240,DKFZP781O2274蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047719,BC047719.1,BM563259,BM563259.1 [Species物種來源]: Homo sapiens 【 TMC7,FLJ21240,DKFZP781O2274蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TMC7,FLJ21240,DKFZP781O2274 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TMC7,FLJ21240,DKFZP781O2274 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TMC7,FLJ21240,DKFZP781O2274 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TMC7,FLJ21240,DKFZP781O2274 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens TMC7,FLJ21240,DKFZP781O2274 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TMC7,FLJ21240,DKFZP781O2274 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TMC7,FLJ21240,DKFZP781O2274 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MGC52110 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MGC52110蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047722,BC047722.1,BM919177,BM919177.1 [Species物種來源]: Homo sapiens 【 MGC52110蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MGC52110 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MGC52110 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MGC52110 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MGC52110 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens MGC52110 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MGC52110 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MGC52110 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
OAF,MGC52117,NS5ATP13TP2 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
OAF,MGC52117,NS5ATP13TP2蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047726,BC047726.1,BM925690,BM925690.1 [Species物種來源]: Homo sapiens 【 OAF,MGC52117,NS5ATP13TP2蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ OAF,MGC52117,NS5ATP13TP2 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 OAF,MGC52117,NS5ATP13TP2 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens OAF,MGC52117,NS5ATP13TP2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 OAF,MGC52117,NS5ATP13TP2 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens OAF,MGC52117,NS5ATP13TP2 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 OAF,MGC52117,NS5ATP13TP2 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 OAF,MGC52117,NS5ATP13TP2 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
DNMT2,PUMET,M.HSAIIP Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
DNMT2,PUMET,M.HSAIIP蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047733,BC047733.1,BQ940333,BQ940333.1 [Species物種來源]: Homo sapiens 【 DNMT2,PUMET,M.HSAIIP蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DNMT2,PUMET,M.HSAIIP Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DNMT2,PUMET,M.HSAIIP Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DNMT2,PUMET,M.HSAIIP protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DNMT2,PUMET,M.HSAIIP Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens DNMT2,PUMET,M.HSAIIP [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DNMT2,PUMET,M.HSAIIP Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DNMT2,PUMET,M.HSAIIP Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MGC12760 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MGC12760蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047736,BC047736.1,BQ953663,BQ953663.1 [Species物種來源]: Homo sapiens 【 MGC12760蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MGC12760 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MGC12760 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MGC12760 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MGC12760 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens MGC12760 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MGC12760 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MGC12760 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
HINT2,HIT-17 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
HINT2,HIT-17蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047737,BC047737.2,BQ218066,BQ218066.1 [Species物種來源]: Homo sapiens 【 HINT2,HIT-17蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HINT2,HIT-17 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HINT2,HIT-17 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HINT2,HIT-17 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HINT2,HIT-17 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens HINT2,HIT-17 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HINT2,HIT-17 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HINT2,HIT-17 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KCNK12 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
KCNK12蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047749,BC047749.1,BQ717909,BQ717909.1 [Species物種來源]: Homo sapiens 【 KCNK12蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KCNK12 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KCNK12 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KCNK12 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KCNK12 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens KCNK12 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KCNK12 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KCNK12 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
REN,FLJ10761 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
REN,FLJ10761蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047752,BC047752.1,BM906275,BM906275.1 [Species物種來源]: Homo sapiens 【 REN,FLJ10761蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ REN,FLJ10761 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 REN,FLJ10761 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens REN,FLJ10761 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 REN,FLJ10761 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens REN,FLJ10761 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 REN,FLJ10761 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 REN,FLJ10761 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047754,BC047754.1,BQ929793,BQ929793.1 [Species物種來源]: Homo sapiens 【 P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 P97,AAG1,DAP5,NAT1,EIF4G2,FLJ41344 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
QC,GCT,QPCT Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
QC,GCT,QPCT蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047756,BC047756.2,BQ232897,BQ232897.1 [Species物種來源]: Homo sapiens 【 QC,GCT,QPCT蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ QC,GCT,QPCT Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 QC,GCT,QPCT Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens QC,GCT,QPCT protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 QC,GCT,QPCT Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens QC,GCT,QPCT [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 QC,GCT,QPCT Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 QC,GCT,QPCT Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MSY2,YBX2,CSDA3,MGC45104 Protein & Antibody, Cell Lysate蛋白/抗體/細胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MSY2,YBX2,CSDA3,MGC45104蛋白/抗體/細胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號]: BC047760,BC047760.1,BM564155,BM564155.1 [Species物種來源]: Homo sapiens 【 MSY2,YBX2,CSDA3,MGC45104蛋白產品信息】 [Protein Construction蛋白構建]: A DNA sequence encoding the Target Protein was expressed. [Tag標簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MSY2,YBX2,CSDA3,MGC45104 Protein QC Testing蛋白質控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導] [Storage儲存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MSY2,YBX2,CSDA3,MGC45104 Antibody Product Information抗體產品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MSY2,YBX2,CSDA3,MGC45104 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MSY2,YBX2,CSDA3,MGC45104 Antibody Usage Guide抗體使用指導】 [Specificity特異性]: Homo sapiens MSY2,YBX2,CSDA3,MGC45104 [Application推薦應用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MSY2,YBX2,CSDA3,MGC45104 Transfected / Overexpression Cell Lysate Product Information細胞裂解液產品信息】 [Expressed Host表達宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MSY2,YBX2,CSDA3,MGC45104 Transfected / Overexpression Cell Lysate Usage Guide細胞裂解液使用指導】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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