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ATP1A1,MGC3285,MGC51750 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ATP1A1,MGC3285,MGC51750蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050359,BC050359.1,BQ213442,BQ213442.1 [Species物種來源]: Homo sapiens 【 ATP1A1,MGC3285,MGC51750蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ATP1A1,MGC3285,MGC51750 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ATP1A1,MGC3285,MGC51750 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ATP1A1,MGC3285,MGC51750 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ATP1A1,MGC3285,MGC51750 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ATP1A1,MGC3285,MGC51750 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ATP1A1,MGC3285,MGC51750 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ATP1A1,MGC3285,MGC51750 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GRTH,DDX25 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
GRTH,DDX25蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050360,BC050360.1,BM554209,BM554209.1 [Species物種來源]: Homo sapiens 【 GRTH,DDX25蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GRTH,DDX25 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GRTH,DDX25 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GRTH,DDX25 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GRTH,DDX25 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GRTH,DDX25 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GRTH,DDX25 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GRTH,DDX25 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MMP19 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MMP19蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050368,BC050368.1,BM924659,BM924659.1 [Species物種來源]: Homo sapiens 【 MMP19蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MMP19 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MMP19 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MMP19 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MMP19 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens MMP19 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MMP19 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MMP19 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
THEDC1,FLJ11106,MGC51852 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
THEDC1,FLJ11106,MGC51852蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050372,BC050372.1,BM554164,BM554164.1 [Species物種來源]: Homo sapiens 【 THEDC1,FLJ11106,MGC51852蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ THEDC1,FLJ11106,MGC51852 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 THEDC1,FLJ11106,MGC51852 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens THEDC1,FLJ11106,MGC51852 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 THEDC1,FLJ11106,MGC51852 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens THEDC1,FLJ11106,MGC51852 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 THEDC1,FLJ11106,MGC51852 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 THEDC1,FLJ11106,MGC51852 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
RNF144 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
RNF144蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050373,BC050373.1,BM546323,BM546323.1 [Species物種來源]: Homo sapiens 【 RNF144蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RNF144 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RNF144 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RNF144 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RNF144 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens RNF144 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RNF144 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RNF144 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
VABP,MGC95,KCHIP1,KCNIP1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
VABP,MGC95,KCHIP1,KCNIP1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050375,BC050375.1,BM920920,BM920920.1 [Species物種來源]: Homo sapiens 【 VABP,MGC95,KCHIP1,KCNIP1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ VABP,MGC95,KCHIP1,KCNIP1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 VABP,MGC95,KCHIP1,KCNIP1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens VABP,MGC95,KCHIP1,KCNIP1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 VABP,MGC95,KCHIP1,KCNIP1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens VABP,MGC95,KCHIP1,KCNIP1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 VABP,MGC95,KCHIP1,KCNIP1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 VABP,MGC95,KCHIP1,KCNIP1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GPX3,GPX-P,GSHPX-3,GSHPX-P Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
GPX3,GPX-P,GSHPX-3,GSHPX-P蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050378,BC050378.1,BM807229,BM807229.1 [Species物種來源]: Homo sapiens 【 GPX3,GPX-P,GSHPX-3,GSHPX-P蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GPX3,GPX-P,GSHPX-3,GSHPX-P Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GPX3,GPX-P,GSHPX-3,GSHPX-P Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GPX3,GPX-P,GSHPX-3,GSHPX-P protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GPX3,GPX-P,GSHPX-3,GSHPX-P Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GPX3,GPX-P,GSHPX-3,GSHPX-P [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GPX3,GPX-P,GSHPX-3,GSHPX-P Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GPX3,GPX-P,GSHPX-3,GSHPX-P Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ST3GAL3 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ST3GAL3蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050380,BC050380.1,BQ228210,BQ228210.1 [Species物種來源]: Homo sapiens 【 ST3GAL3蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ST3GAL3 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ST3GAL3 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ST3GAL3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ST3GAL3 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ST3GAL3 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ST3GAL3 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ST3GAL3 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PLCD1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
PLCD1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050382,BC050382.1,BQ228056,BQ228056.1 [Species物種來源]: Homo sapiens 【 PLCD1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PLCD1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PLCD1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PLCD1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PLCD1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PLCD1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PLCD1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PLCD1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SPT3,SPT3L,SUPT3H Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SPT3,SPT3L,SUPT3H蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050384,BC050384.1,BM923256,BM923256.1 [Species物種來源]: Homo sapiens 【 SPT3,SPT3L,SUPT3H蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SPT3,SPT3L,SUPT3H Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SPT3,SPT3L,SUPT3H Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SPT3,SPT3L,SUPT3H protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SPT3,SPT3L,SUPT3H Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens SPT3,SPT3L,SUPT3H [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SPT3,SPT3L,SUPT3H Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SPT3,SPT3L,SUPT3H Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ANKS3,FLJ43261,LOC146562 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ANKS3,FLJ43261,LOC146562蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050387,BC050387.1,BQ067328,BQ067328.1 [Species物種來源]: Homo sapiens 【 ANKS3,FLJ43261,LOC146562蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ANKS3,FLJ43261,LOC146562 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ANKS3,FLJ43261,LOC146562 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ANKS3,FLJ43261,LOC146562 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ANKS3,FLJ43261,LOC146562 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ANKS3,FLJ43261,LOC146562 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ANKS3,FLJ43261,LOC146562 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ANKS3,FLJ43261,LOC146562 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050389,BC050389.1,BM922843,BM922843.1 [Species物種來源]: Homo sapiens 【 GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GP2B,CD41B,MGRN1,ITGA2B,RNF156,KIAA0544 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FBX25,FBXO25,MGC20256,MGC51975蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050393,BC050393.1,BQ219014,BQ219014.1 [Species物種來源]: Homo sapiens 【 FBX25,FBXO25,MGC20256,MGC51975蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FBX25,FBXO25,MGC20256,MGC51975 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FBX25,FBXO25,MGC20256,MGC51975 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FBX25,FBXO25,MGC20256,MGC51975 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FBX25,FBXO25,MGC20256,MGC51975 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FBX25,FBXO25,MGC20256,MGC51975 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FBX25,FBXO25,MGC20256,MGC51975 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FBX25,FBXO25,MGC20256,MGC51975 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
C14ORF54,FLJ36130 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C14ORF54,FLJ36130蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050401,BC050401.1,BM908047,BM908047.1 [Species物種來源]: Homo sapiens 【 C14ORF54,FLJ36130蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C14ORF54,FLJ36130 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C14ORF54,FLJ36130 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C14ORF54,FLJ36130 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C14ORF54,FLJ36130 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens C14ORF54,FLJ36130 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C14ORF54,FLJ36130 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C14ORF54,FLJ36130 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PTER,RPR-1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
PTER,RPR-1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050411,BC050411.1,BQ937707,BQ937707.1 [Species物種來源]: Homo sapiens 【 PTER,RPR-1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PTER,RPR-1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PTER,RPR-1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PTER,RPR-1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PTER,RPR-1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PTER,RPR-1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PTER,RPR-1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PTER,RPR-1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050414,BC050414.1,BQ923710,BQ923710.1 [Species物種來源]: Homo sapiens 【 ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ADRA2,ADRAR,ZNF32,ADRA2A,ADRA2R,ALPHA2AAR Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF322A Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZNF322A蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050425,BC050425.1,BQ932352,BQ932352.1 [Species物種來源]: Homo sapiens 【 ZNF322A蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF322A Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF322A Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF322A protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF322A Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ZNF322A [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF322A Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF322A Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
RAB17,FLJ12538 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
RAB17,FLJ12538蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050426,BC050426.1,BM924743,BM924743.1 [Species物種來源]: Homo sapiens 【 RAB17,FLJ12538蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RAB17,FLJ12538 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RAB17,FLJ12538 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RAB17,FLJ12538 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RAB17,FLJ12538 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens RAB17,FLJ12538 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RAB17,FLJ12538 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RAB17,FLJ12538 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050428,BC050428.1,BM926138,BM926138.1 [Species物種來源]: Homo sapiens 【 KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KATNA1,C6ORF95,ZC3H12D,FLJ46041,DJ281H8.1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CPT,CPT1,CHPT1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CPT,CPT1,CHPT1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC050429,BC050429.1,BQ923737,BQ923737.1 [Species物種來源]: Homo sapiens 【 CPT,CPT1,CHPT1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CPT,CPT1,CHPT1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CPT,CPT1,CHPT1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CPT,CPT1,CHPT1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CPT,CPT1,CHPT1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CPT,CPT1,CHPT1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CPT,CPT1,CHPT1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CPT,CPT1,CHPT1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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