- 抗體共 211329 條
LOC401127 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
LOC401127蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010526,BC010526.2,BF343965,BF343965.1 [Species物種來源]: Homo sapiens 【 LOC401127蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ LOC401127 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 LOC401127 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens LOC401127 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 LOC401127 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens LOC401127 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 LOC401127 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 LOC401127 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010530,BC010530.1,BF341911,BF341911.1 [Species物種來源]: Homo sapiens 【 ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ACT,AACT,GIG24,GIG25,MGC88254,SERPINA3 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
C18ORF18 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C18ORF18蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010538,BC010538.1,BE543780,BE543780.1 [Species物種來源]: Homo sapiens 【 C18ORF18蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C18ORF18 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C18ORF18 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C18ORF18 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C18ORF18 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens C18ORF18 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C18ORF18 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C18ORF18 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ANG2,C11ORF2 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ANG2,C11ORF2蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010540,BC010540.1,BE547478,BE547478.1 [Species物種來源]: Homo sapiens 【 ANG2,C11ORF2蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ANG2,C11ORF2 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ANG2,C11ORF2 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ANG2,C11ORF2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ANG2,C11ORF2 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ANG2,C11ORF2 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ANG2,C11ORF2 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ANG2,C11ORF2 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
AAG13,S100F,DT1P1A7,S100A16,MGC17528蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010541,BC010541.1,BE547108,BE547108.1 [Species物種來源]: Homo sapiens 【 AAG13,S100F,DT1P1A7,S100A16,MGC17528蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AAG13,S100F,DT1P1A7,S100A16,MGC17528 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AAG13,S100F,DT1P1A7,S100A16,MGC17528 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AAG13,S100F,DT1P1A7,S100A16,MGC17528 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AAG13,S100F,DT1P1A7,S100A16,MGC17528 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens AAG13,S100F,DT1P1A7,S100A16,MGC17528 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AAG13,S100F,DT1P1A7,S100A16,MGC17528 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AAG13,S100F,DT1P1A7,S100A16,MGC17528 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MED8,ARC32,MGC17544,MGC19641 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MED8,ARC32,MGC17544,MGC19641蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010543,BC010543.1,BE549120,BE549120.1 [Species物種來源]: Homo sapiens 【 MED8,ARC32,MGC17544,MGC19641蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MED8,ARC32,MGC17544,MGC19641 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MED8,ARC32,MGC17544,MGC19641 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MED8,ARC32,MGC17544,MGC19641 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MED8,ARC32,MGC17544,MGC19641 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens MED8,ARC32,MGC17544,MGC19641 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MED8,ARC32,MGC17544,MGC19641 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MED8,ARC32,MGC17544,MGC19641 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZPR9,ZNF622,MGC2485,MGC17552 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZPR9,ZNF622,MGC2485,MGC17552蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010545,BC010545.1,BE544624,BE544624.1 [Species物種來源]: Homo sapiens 【 ZPR9,ZNF622,MGC2485,MGC17552蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZPR9,ZNF622,MGC2485,MGC17552 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZPR9,ZNF622,MGC2485,MGC17552 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZPR9,ZNF622,MGC2485,MGC17552 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZPR9,ZNF622,MGC2485,MGC17552 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ZPR9,ZNF622,MGC2485,MGC17552 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZPR9,ZNF622,MGC2485,MGC17552 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZPR9,ZNF622,MGC2485,MGC17552 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
NCE2,UBE2F,MGC18120 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
NCE2,UBE2F,MGC18120蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010549,BC010549.1,BF345978,BF345978.1 [Species物種來源]: Homo sapiens 【 NCE2,UBE2F,MGC18120蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NCE2,UBE2F,MGC18120 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NCE2,UBE2F,MGC18120 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NCE2,UBE2F,MGC18120 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NCE2,UBE2F,MGC18120 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens NCE2,UBE2F,MGC18120 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NCE2,UBE2F,MGC18120 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NCE2,UBE2F,MGC18120 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
STAR,STARD1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
STAR,STARD1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010550,BC010550.1,BF345799,BF345799.1 [Species物種來源]: Homo sapiens 【 STAR,STARD1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ STAR,STARD1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 STAR,STARD1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens STAR,STARD1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 STAR,STARD1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens STAR,STARD1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 STAR,STARD1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 STAR,STARD1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TIMP4 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
TIMP4蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010553,BC010553.1,BF346229,BF346229.1 [Species物種來源]: Homo sapiens 【 TIMP4蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TIMP4 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TIMP4 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TIMP4 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TIMP4 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens TIMP4 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TIMP4 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TIMP4 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010556,BC010556.1,BF345389,BF345389.1 [Species物種來源]: Homo sapiens 【 HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HAP,RTN3,ASYIP,NSPL2,NSPLII,RTN3-A1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FAM107A Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
FAM107A蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010561,BC010561.1,BF342045,BF342045.1 [Species物種來源]: Homo sapiens 【 FAM107A蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FAM107A Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FAM107A Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FAM107A protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FAM107A Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FAM107A [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FAM107A Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FAM107A Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
C16ORF14,MGC15416,C349E10.1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
C16ORF14,MGC15416,C349E10.1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010562,BC010562.1,BF344087,BF344087.1 [Species物種來源]: Homo sapiens 【 C16ORF14,MGC15416,C349E10.1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C16ORF14,MGC15416,C349E10.1 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C16ORF14,MGC15416,C349E10.1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C16ORF14,MGC15416,C349E10.1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C16ORF14,MGC15416,C349E10.1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens C16ORF14,MGC15416,C349E10.1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C16ORF14,MGC15416,C349E10.1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C16ORF14,MGC15416,C349E10.1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ADAMTS6,ADAM-TS6 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ADAMTS6,ADAM-TS6蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010563,BC010563.1,BF344144,BF344144.1 [Species物種來源]: Homo sapiens 【 ADAMTS6,ADAM-TS6蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ADAMTS6,ADAM-TS6 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ADAMTS6,ADAM-TS6 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ADAMTS6,ADAM-TS6 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ADAMTS6,ADAM-TS6 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ADAMTS6,ADAM-TS6 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ADAMTS6,ADAM-TS6 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ADAMTS6,ADAM-TS6 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
VIPR2,FLJ16511 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
VIPR2,FLJ16511蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010569,BC010569.1,BF527866,BF527866.1 [Species物種來源]: Homo sapiens 【 VIPR2,FLJ16511蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ VIPR2,FLJ16511 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 VIPR2,FLJ16511 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens VIPR2,FLJ16511 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 VIPR2,FLJ16511 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens VIPR2,FLJ16511 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 VIPR2,FLJ16511 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 VIPR2,FLJ16511 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
HL,HMGCL Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
HL,HMGCL蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010570,BC010570.1,BF527971,BF527971.1 [Species物種來源]: Homo sapiens 【 HL,HMGCL蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HL,HMGCL Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HL,HMGCL Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HL,HMGCL protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HL,HMGCL Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens HL,HMGCL [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HL,HMGCL Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HL,HMGCL Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GLUR2,GLURB,GRIA2,HBGR2 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
GLUR2,GLURB,GRIA2,HBGR2蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010574,BC010574.1,BF531018,BF531018.1 [Species物種來源]: Homo sapiens 【 GLUR2,GLURB,GRIA2,HBGR2蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GLUR2,GLURB,GRIA2,HBGR2 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GLUR2,GLURB,GRIA2,HBGR2 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GLUR2,GLURB,GRIA2,HBGR2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GLUR2,GLURB,GRIA2,HBGR2 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GLUR2,GLURB,GRIA2,HBGR2 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GLUR2,GLURB,GRIA2,HBGR2 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GLUR2,GLURB,GRIA2,HBGR2 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
AP-4,TFAP4 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
AP-4,TFAP4蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010576,BC010576.1,BF529726,BF529726.1 [Species物種來源]: Homo sapiens 【 AP-4,TFAP4蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AP-4,TFAP4 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AP-4,TFAP4 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AP-4,TFAP4 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AP-4,TFAP4 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens AP-4,TFAP4 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AP-4,TFAP4 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AP-4,TFAP4 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GEP,GRN,GP88,PEPI,PCDGF Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
GEP,GRN,GP88,PEPI,PCDGF蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010577,BC010577.2,BE548835,BE548835.1 [Species物種來源]: Homo sapiens 【 GEP,GRN,GP88,PEPI,PCDGF蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GEP,GRN,GP88,PEPI,PCDGF Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GEP,GRN,GP88,PEPI,PCDGF Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GEP,GRN,GP88,PEPI,PCDGF protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GEP,GRN,GP88,PEPI,PCDGF Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GEP,GRN,GP88,PEPI,PCDGF [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GEP,GRN,GP88,PEPI,PCDGF Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GEP,GRN,GP88,PEPI,PCDGF Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TARS,THRRS,MGC9344 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
TARS,THRRS,MGC9344蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC010578,BC010578.1,BE546830,BE546830.1 [Species物種來源]: Homo sapiens 【 TARS,THRRS,MGC9344蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TARS,THRRS,MGC9344 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TARS,THRRS,MGC9344 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TARS,THRRS,MGC9344 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TARS,THRRS,MGC9344 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens TARS,THRRS,MGC9344 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TARS,THRRS,MGC9344 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TARS,THRRS,MGC9344 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
- 快速發(fā)布求購(gòu)信息
