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CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028199,BC028199.1,BI768733,BI768733.1 [Species物種來源]: Homo sapiens 【 CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CLM1,NKIR,IREM1,CD300F,IGSF13,CD300LF Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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BUB1,BUB1A,BUB1L,HBUB1 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

BUB1,BUB1A,BUB1L,HBUB1蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028201,BC028201.1,BI772303,BI772303.1 [Species物種來源]: Homo sapiens 【 BUB1,BUB1A,BUB1L,HBUB1蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ BUB1,BUB1A,BUB1L,HBUB1 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 BUB1,BUB1A,BUB1L,HBUB1 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens BUB1,BUB1A,BUB1L,HBUB1 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 BUB1,BUB1A,BUB1L,HBUB1 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens BUB1,BUB1A,BUB1L,HBUB1 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 BUB1,BUB1A,BUB1L,HBUB1 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 BUB1,BUB1A,BUB1L,HBUB1 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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PER,HPER,PER1,RIGUI,MGC88021 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

PER,HPER,PER1,RIGUI,MGC88021蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028207,BC028207.1,BI909641,BI909641.1 [Species物種來源]: Homo sapiens 【 PER,HPER,PER1,RIGUI,MGC88021蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PER,HPER,PER1,RIGUI,MGC88021 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PER,HPER,PER1,RIGUI,MGC88021 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PER,HPER,PER1,RIGUI,MGC88021 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PER,HPER,PER1,RIGUI,MGC88021 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PER,HPER,PER1,RIGUI,MGC88021 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PER,HPER,PER1,RIGUI,MGC88021 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PER,HPER,PER1,RIGUI,MGC88021 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028212,BC028212.1,BI907590,BI907590.1 [Species物種來源]: Homo sapiens 【 FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FOAP-2,PIK3R5,P101-PI3K,F730038I15RIK Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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SLC7A6,FLJ13291,SLC7A6OS Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

SLC7A6,FLJ13291,SLC7A6OS蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028216,BC028216.1,BI909342,BI909342.1 [Species物種來源]: Homo sapiens 【 SLC7A6,FLJ13291,SLC7A6OS蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SLC7A6,FLJ13291,SLC7A6OS Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SLC7A6,FLJ13291,SLC7A6OS Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SLC7A6,FLJ13291,SLC7A6OS protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SLC7A6,FLJ13291,SLC7A6OS Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens SLC7A6,FLJ13291,SLC7A6OS [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SLC7A6,FLJ13291,SLC7A6OS Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SLC7A6,FLJ13291,SLC7A6OS Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028219,BC028219.1,BI910817,BI910817.1 [Species物種來源]: Homo sapiens 【 TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TGN38,TGN46,TGN48,TGN51,TTGN2,TGOLN2,MGC14722 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

GRIP1,LGALS12,GALECTIN-12 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

GRIP1,LGALS12,GALECTIN-12蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028222,BC028222.1,BI910052,BI910052.1 [Species物種來源]: Homo sapiens 【 GRIP1,LGALS12,GALECTIN-12蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GRIP1,LGALS12,GALECTIN-12 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GRIP1,LGALS12,GALECTIN-12 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GRIP1,LGALS12,GALECTIN-12 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GRIP1,LGALS12,GALECTIN-12 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GRIP1,LGALS12,GALECTIN-12 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GRIP1,LGALS12,GALECTIN-12 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GRIP1,LGALS12,GALECTIN-12 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

OTUD5,MGC104871,DKFZP761A052 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

OTUD5,MGC104871,DKFZP761A052蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028225,BC028225.1,BI915414,BI915414.1 [Species物種來源]: Homo sapiens 【 OTUD5,MGC104871,DKFZP761A052蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ OTUD5,MGC104871,DKFZP761A052 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 OTUD5,MGC104871,DKFZP761A052 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens OTUD5,MGC104871,DKFZP761A052 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 OTUD5,MGC104871,DKFZP761A052 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens OTUD5,MGC104871,DKFZP761A052 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 OTUD5,MGC104871,DKFZP761A052 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 OTUD5,MGC104871,DKFZP761A052 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028233,BC028233.1,BI914442,BI914442.1 [Species物種來源]: Homo sapiens 【 ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ARF1GAP,ARFGAP1,MGC39924,HRIHFB2281 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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BMP3B,GDF10,BMP-3B Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心

BMP3B,GDF10,BMP-3B蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028237,BC028237.1,BI919076,BI919076.1 [Species物種來源]: Homo sapiens 【 BMP3B,GDF10,BMP-3B蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ BMP3B,GDF10,BMP-3B Protein QC Testing蛋白質(zhì)控檢測] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 BMP3B,GDF10,BMP-3B Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens BMP3B,GDF10,BMP-3B protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 BMP3B,GDF10,BMP-3B Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens BMP3B,GDF10,BMP-3B [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 BMP3B,GDF10,BMP-3B Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 BMP3B,GDF10,BMP-3B Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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