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INPP4A Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
INPP4A蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028361,BC028361.2,BG721993,BG721993.1 [Species物種來源]: Homo sapiens 【 INPP4A蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ INPP4A Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 INPP4A Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens INPP4A protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 INPP4A Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens INPP4A [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 INPP4A Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 INPP4A Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MCD4,MDC4,PIGN,PIG-N,MGC26427蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028363,BC028363.2,BG722695,BG722695.1 [Species物種來源]: Homo sapiens 【 MCD4,MDC4,PIGN,PIG-N,MGC26427蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MCD4,MDC4,PIGN,PIG-N,MGC26427 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MCD4,MDC4,PIGN,PIG-N,MGC26427 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MCD4,MDC4,PIGN,PIG-N,MGC26427 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MCD4,MDC4,PIGN,PIG-N,MGC26427 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens MCD4,MDC4,PIGN,PIG-N,MGC26427 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MCD4,MDC4,PIGN,PIG-N,MGC26427 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MCD4,MDC4,PIGN,PIG-N,MGC26427 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FBXW10 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
FBXW10蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028364,BC028364.2,BG721464,BG721464.1 [Species物種來源]: Homo sapiens 【 FBXW10蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FBXW10 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FBXW10 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FBXW10 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FBXW10 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FBXW10 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FBXW10 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FBXW10 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
MGC26647 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
MGC26647蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028365,BC028365.2,BG773400,BG773400.1 [Species物種來源]: Homo sapiens 【 MGC26647蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MGC26647 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MGC26647 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MGC26647 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MGC26647 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens MGC26647 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MGC26647 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MGC26647 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CEP4L,TSGA10 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
CEP4L,TSGA10蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028366,BC028366.1,BG722030,BG722030.1 [Species物種來源]: Homo sapiens 【 CEP4L,TSGA10蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CEP4L,TSGA10 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CEP4L,TSGA10 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CEP4L,TSGA10 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CEP4L,TSGA10 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CEP4L,TSGA10 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CEP4L,TSGA10 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CEP4L,TSGA10 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF564 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZNF564蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028367,BC028367.1,BG771726,BG771726.1 [Species物種來源]: Homo sapiens 【 ZNF564蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF564 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF564 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF564 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF564 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ZNF564 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF564 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF564 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028369,BC028369.2,BG723401,BG723401.1 [Species物種來源]: Homo sapiens 【 FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FEM1A,FEM1C,KIAA1785,EUROIMAGE686608,EUROIMAGE783647 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028371,BC028371.1,BG718583,BG718583.1 [Species物種來源]: Homo sapiens 【 GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GLB1L,PSMA8,PSMA7L,MGC10771,MGC26605 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SVPH4,ADAM30 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SVPH4,ADAM30蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028372,BC028372.2,BG718870,BG718870.1 [Species物種來源]: Homo sapiens 【 SVPH4,ADAM30蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SVPH4,ADAM30 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SVPH4,ADAM30 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SVPH4,ADAM30 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SVPH4,ADAM30 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens SVPH4,ADAM30 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SVPH4,ADAM30 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SVPH4,ADAM30 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TRM10,RG9MTD2,FLJ20325,MGC27034蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028373,BC028373.2,BG772206,BG772206.1 [Species物種來源]: Homo sapiens 【 TRM10,RG9MTD2,FLJ20325,MGC27034蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TRM10,RG9MTD2,FLJ20325,MGC27034 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TRM10,RG9MTD2,FLJ20325,MGC27034 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TRM10,RG9MTD2,FLJ20325,MGC27034 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TRM10,RG9MTD2,FLJ20325,MGC27034 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens TRM10,RG9MTD2,FLJ20325,MGC27034 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TRM10,RG9MTD2,FLJ20325,MGC27034 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TRM10,RG9MTD2,FLJ20325,MGC27034 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028375,BC028375.1,BG773012,BG773012.1 [Species物種來源]: Homo sapiens 【 PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PDZD8,PDZK8,FLJ25412,FLJ34427,BA129M16.2 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF502,FLJ12515,FLJ14855 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ZNF502,FLJ12515,FLJ14855蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028377,BC028377.2,BG719390,BG719390.1 [Species物種來源]: Homo sapiens 【 ZNF502,FLJ12515,FLJ14855蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF502,FLJ12515,FLJ14855 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF502,FLJ12515,FLJ14855 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF502,FLJ12515,FLJ14855 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF502,FLJ12515,FLJ14855 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ZNF502,FLJ12515,FLJ14855 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF502,FLJ12515,FLJ14855 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF502,FLJ12515,FLJ14855 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SYT3,SYTIII,DKFZP761O132 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
SYT3,SYTIII,DKFZP761O132蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028379,BC028379.1,BG704063,BG704063.1 [Species物種來源]: Homo sapiens 【 SYT3,SYTIII,DKFZP761O132蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SYT3,SYTIII,DKFZP761O132 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SYT3,SYTIII,DKFZP761O132 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SYT3,SYTIII,DKFZP761O132 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SYT3,SYTIII,DKFZP761O132 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens SYT3,SYTIII,DKFZP761O132 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SYT3,SYTIII,DKFZP761O132 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SYT3,SYTIII,DKFZP761O132 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ALK3,CD292,BMPR1A,ACVRLK3 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
ALK3,CD292,BMPR1A,ACVRLK3蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028383,BC028383.1,BG720615,BG720615.1 [Species物種來源]: Homo sapiens 【 ALK3,CD292,BMPR1A,ACVRLK3蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ALK3,CD292,BMPR1A,ACVRLK3 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ALK3,CD292,BMPR1A,ACVRLK3 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ALK3,CD292,BMPR1A,ACVRLK3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ALK3,CD292,BMPR1A,ACVRLK3 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens ALK3,CD292,BMPR1A,ACVRLK3 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ALK3,CD292,BMPR1A,ACVRLK3 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ALK3,CD292,BMPR1A,ACVRLK3 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028384,BC028384.2,BG706468,BG706468.1 [Species物種來源]: Homo sapiens 【 GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GUCA3,GC-SA3,GUC1A3,GUCSA3,GUCY1A3 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028385,BC028385.2,BG702643,BG702643.1 [Species物種來源]: Homo sapiens 【 CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CYC4,CYP60,PPIL2,MGC787,HCYP-60,FLJ39930,MGC33174 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KIAA0179 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
KIAA0179蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028386,BC028386.2,BG716976,BG716976.1 [Species物種來源]: Homo sapiens 【 KIAA0179蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KIAA0179 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KIAA0179 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KIAA0179 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KIAA0179 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens KIAA0179 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KIAA0179 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KIAA0179 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
DMR,WDR20,FLJ33659,MGC33177,MGC33183蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028387,BC028387.2,BG720159,BG720159.1 [Species物種來源]: Homo sapiens 【 DMR,WDR20,FLJ33659,MGC33177,MGC33183蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DMR,WDR20,FLJ33659,MGC33177,MGC33183 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DMR,WDR20,FLJ33659,MGC33177,MGC33183 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DMR,WDR20,FLJ33659,MGC33177,MGC33183 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DMR,WDR20,FLJ33659,MGC33177,MGC33183 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens DMR,WDR20,FLJ33659,MGC33177,MGC33183 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DMR,WDR20,FLJ33659,MGC33177,MGC33183 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DMR,WDR20,FLJ33659,MGC33177,MGC33183 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
IMP4,PSL1,SPPL2B,KIAA1532 Protein & Antibody, Cell Lysate蛋白/抗體/細(xì)胞裂解液-BioVector NTCC基因蛋白抗體保藏中心
IMP4,PSL1,SPPL2B,KIAA1532蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028391,BC028391.2,BG719434,BG719434.1 [Species物種來源]: Homo sapiens 【 IMP4,PSL1,SPPL2B,KIAA1532蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ IMP4,PSL1,SPPL2B,KIAA1532 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 IMP4,PSL1,SPPL2B,KIAA1532 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens IMP4,PSL1,SPPL2B,KIAA1532 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 IMP4,PSL1,SPPL2B,KIAA1532 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens IMP4,PSL1,SPPL2B,KIAA1532 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 IMP4,PSL1,SPPL2B,KIAA1532 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 IMP4,PSL1,SPPL2B,KIAA1532 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415蛋白/抗體/細(xì)胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因編號(hào)]: BC028393,BC028393.2,BG720983,BG720983.1 [Species物種來源]: Homo sapiens 【 AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415蛋白產(chǎn)品信息】 [Protein Construction蛋白構(gòu)建]: A DNA sequence encoding the Target Protein was expressed. [Tag標(biāo)簽]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子種屬]: Homo sapiens [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping運(yùn)輸說明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 Protein QC Testing蛋白質(zhì)控檢測(cè)] Purity純度: > 97 % as determined by SDS-PAGE Endotoxin內(nèi)毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation緩沖液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指導(dǎo)] [Storage儲(chǔ)存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution復(fù)溶條件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 Antibody Product Information抗體產(chǎn)品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 protein [Ig Type Ig類型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation緩沖液]: 0.2 μm filtered solution in PBS [Preparation抗體制備方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 Antibody Usage Guide抗體使用指導(dǎo)】 [Specificity特異性]: Homo sapiens AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 [Application推薦應(yīng)用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage儲(chǔ)存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 Transfected / Overexpression Cell Lysate Product Information細(xì)胞裂解液產(chǎn)品信息】 [Expressed Host表達(dá)宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description產(chǎn)品介紹]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AMAP1,AMAP-1,MYCBPAP,DKFZP434N1415 Transfected / Overexpression Cell Lysate Usage Guide細(xì)胞裂解液使用指導(dǎo)】 [Preparation Method制備方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解緩沖液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing質(zhì)控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability穩(wěn)定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建議]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer儲(chǔ)存緩沖液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction儲(chǔ)存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes應(yīng)用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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