| VK2/E6E7 |
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| | D Anderson, RN Fichorova, JG Rheinwald |
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| | Biosafety Level: | 2 [Cells contain human Papilloma viral sequences ] |
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| | frozen |
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| | See Propagation |
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| | adherent |
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| | Homo sapiens deposited as human |
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| | epithelial
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| | Organ: vagina
Tissue: mucosa
Cell Type: epithelialHPV-16 E6/E7 transformed |
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| | cytokeratins 8 (CK8), 10 (CK10), 13 (CK13), 18 (CK18) and 19 (CK19) [52983 ]
macrophage colony-stimulating factor (M-CSF); transforming growth factor beta1; interleukin 8 (IL-8); prostaglandin E2; the secretory leukoproteinase inhibitor; polymeric immunoglobulin receptor [52984 ] |
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| | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Deborah Anderson of The Brigham and Women's Hospital, Inc. and is provided for academic research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes, including screening of compounds. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied. 2. All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express written permission from the The Brigham and Women's Hospital, Inc. 3. Any proposed commercial manufacture, use, or sale of the these cells, or their products must first be approved and the terms for such commercial manufacture, use, or sale must first be negotiated with TheBrigham and Women's Hospital, Inc., Office of Corporate Sponsored Research and Licensing, 75 Francis Street, Boston, MA 02115 (617) 525-6010. |
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| | Isolation date: 1996 |
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| | Amelogenin: X
CSF1PO: 10, 11
D5S818: 9, 10
D13S317: 9, 12
D7S820: 10, 11
D16S539: 9
vWA: 16
THO1: 7, 9.3
TPOX: 11 |
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| | 32 years adult |
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| | female |
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| | Propagation: | ATCC complete growth medium: Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 0.1 ng/ml human recombinant EGF, 0.05 mg/ml bovine pituitary extract, and additional calcium chloride 44.1 mg/L (final concentration 0.4 mM)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
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| | Protocol: The cells should not be allowed to become confluent, subculture at 60 to 90% of confluence. Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Neutralize the trypsin by adding a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 10% fetal bovine serum. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh serum-free growth medium, aspirate and dispense into new flasks. Cells will not attach well for 24 hours after subculture.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days |
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| | Freeze medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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| | 24 hrs |
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| | 52983: Fichorova RN, et al. Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins. Biol. Reprod. 57: 847-855, 1999. PubMed: 9314589
52984: Fichorova RN, Anderson DJ. Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells. Biol. Reprod. 60: 508-514, 1999. PubMed: 9916021
53415: Fichorova RN, et al. Distinct proinflammatory host responses to Neisseria gonorrhoeae infection in immortalized human cervical and vaginal epithelial cells. Infect. Immun. 69: 5840-5880, 2001. PubMed:11500462
53416: Fichorova RN, et al. The molecular basis of nonoxynol-9-induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission. J. Infect. Dis. 184: 418-428, 2001. PubMed:11471099 |
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