pLenti6.3/V5-DEST慢病毒表達載體質粒圖譜序列抗性價格報價Biovector NTCC Inc.
- 價 格:¥1920
- 貨 號:BiovectorLenti6.3/V5-DEST
- 產 地:北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作QQ:1843439339 (微信同號)
郵件:Biovector@163.com
手機:18901268599
地址:北京
- 已注冊
載體基本信息
出品公司: | Invitrogen |
---|---|
載體名稱: | pLenti6.3/V5-DEST |
質粒類型: | 慢病毒表達載體;Gateway系統(tǒng)載體 |
高拷貝/低拷貝: | 高拷貝 |
啟動子: | CMV |
克隆方法: | Gateway |
載體大小: | 9387 bp |
5' 測序引物及序列: | CMV-F |
3' 測序引物及序列: | WPRE-R |
載體標簽: | V5 Epitope Tag |
載體抗性: | Ampicillin |
篩選標記: | Blasticidin |
備注: | The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway? destination vector for expression of a C- terminally V5-tagged protein. |
產品目錄號: | V533-06 |
穩(wěn)定性: | 穩(wěn)表達 |
組成型: | 組成型 |
病毒/非病毒: | 慢病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
DescriptionInvitrogen? 's pLenti6.3?V5-DEST? Gateway? Vector Kit is part of our ViraPower? HiPerform? Lentiviral Gateway? Expression Kit (catalog K5330-00). The pLenti6.3?V5-DEST? Gateway? Vector Kit contains the Gateway?-adapted ViraPower? HiPerform? lentiviral expression vector, pLenti6.3?V5-DEST? for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3?V5-DEST? vector is equipped with two key genetic elements, making it a HiPerform? vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.Advantages? Stable expression? Long-term experiments? Accurate titer of functional virus? Flexible and versatile Gateway? recombination cloning technologyKey Features? HiPerform? WPRE and cPPT elements? CMV promoter ? V5 epitope tag at C terminus? Blasticidin selection
載體序列
載體質粒圖譜pdf版和相關資料下載
載體應用舉例
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