LOCUS       pAN7-1                  6756 bp    DNA     circular   SYN 14-NOV-2006
DEFINITION  Expression vector (PAN7-1) DNA, 6756bp.
ACCESSION   Z32698
VERSION     Z32698.1  GI:475166
KEYWORDS    hygromycin b phosphotransferase.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
REFERENCE   1
  AUTHORS   Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and
            Timberlake,W.E.
  TITLE     Primary structure of the trpC gene from Aspergillus nidulans
  JOURNAL   Mol. Gen. Genet. 199 (1), 37-45 (1985)
   PUBMED   3158796
  REMARK    sites .
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
     misc_feature    1..6756
                     /product="fungal transformation vector pAN7-1"
                     /standard_name="HPH"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /phenotype="gene product confers resistance to hygromycin
                     B"
     promoter        1..2129
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /citation=[3]
                     /function="promoter region of the A.nidulans gpdA gene"
     gene            2130
                     /gene="gpdA"
     misc_feature    2130
                     /gene="gpdA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[3]
                     /function="major transcription start site"
     intron          2178..2293
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[3]
                     /number=1
     gene            2302..3321
                     /gene="HPH"
     CDS             2302..3321
                     /gene="HPH"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /label="gene-product confers resistance to hygromycin B"
                     /citation=[2]
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin B phosphotransferase"
                     /protein_id="CAA83647.1"
                     /db_xref="GI:475167"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEA
                     MFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
                     VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTR
                     PRAKE"
     terminator      3339..4108
                     /standard_name="terminator region from A.nidulans trpC
                     gene (containing CDS)"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /label="this region contains the 3'end of the trpC
                     transcript including 50 codons. two major transcription
                     ends have been identified"
                     /citation=[1]
                     /citation=[2]
     polyA_site      3590..3600
     misc_difference 3600..3610
                     /label="introduction of one base at this position generates
                     an BLGI site with was shown to be present by restriction
                     digestion"
                     /citation=[1]
                     /replace="gccttncaggc"
     misc_difference 3809..3817
                     /label="introduction of one base at this position generates
                     an ALWNI site which was shown to be present by restriction
                     digestion"
                     /citation=[1]
                     /replace="cagcncctg"
     polyA_site      3863..3873
     misc_difference 3897..3907
                     /label="introduction (actual sequence could be deletion as
                     well) of a base at this position results in the
                     elimination of an XMNI site which was shown to be absent
                     by restriction digestion"
                     /citation=[1]
                     /replace="gaaatcanttc"
     variation       3934..3941
                     /label="A NCOI site present at this position was removed by
                     digestion, treatment with klenow polymerase and
                     religation"
                     /citation=[2]
                     /replace="catgcatg"
     misc_feature    4105..6756
                     /product="PUC18"
                     /label="vector sequences derived from PUC18 from SALI TO
                     ECORI"
                     /citation=[2]
ORIGIN      
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     2281 cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     2401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     2521 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     2581 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     2641 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     2701 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     2761 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     2821 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     2881 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     2941 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3001 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3061 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3121 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3181 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3241 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3301 cgtccgaggg caaaggaata gagtagatgc cgaccgcggg atccacttaa cgttactgaa
     3361 atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
     3421 gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
     3481 agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt tttcgggttt
     3541 acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa cctagtaacg
     3601 ccttncaggc tccggcgaag agaagaatag cttagcagag ctattttcat tttcgggaga
     3661 cgagatcaag cagatcaacg gtcgtcaaga gacctacgag actgaggaat ccgctcttgg
     3721 ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct tctttactct
     3781 gatagcttga ctatgaaaat tccgtcacca gcncctgggt tcgcaaagat aattgcatgt
     3841 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
     3901 tcanttccta ctaagatggt atacaatagt aaccatgcat ggttgcctag tgaatgctcc
     3961 gtaacaccca atacgccggc cgaaactttt ttacaactct cctatgagtc gtttacccag
     4021 aatgcacagg tacacttgtt tagaggtaat ccttctttct agaagtcctc gtgtactgtg
     4081 taagcgccca ctccacatct ccactcgacc tgcaggcatg caagcttggc actggccgtc
     4141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4261 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     4321 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4381 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4441 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4501 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4561 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4621 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4681 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4741 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4801 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4861 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4921 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4981 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5041 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5101 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5161 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5221 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5281 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5341 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5401 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5461 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5521 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5581 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5641 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5701 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5761 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5821 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5881 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5941 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6001 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6061 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6121 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6181 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6241 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6301 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6361 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6421 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6481 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6541 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6601 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6661 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6721 caatttcaca caggaaacag ctatgaccat gattac
//