pgRNA-bacteria細菌CRISPR/Cas9載體質粒，表達guide RNA，用于細菌基因敲除-BioVector NTCC保藏中心
- 價 格：￥2930
- 貨 號：pgRNA-bacteria
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
pgRNA-bacteria
(BioVector-044251)
細菌CRISPR/Cas9載體質粒，表達guide RNA，用于細菌基因敲除。
配套質粒：
pdCas9-bacteria
pwtCas9-bacteria
pCRISPR
pCas9
pdCas9
- PURPOSEExpression of customizable guide RNA (gRNA) for bacterial gene knockdown
- DEPOSITING LABStanley Qi
- PUBLICATIONQi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
BACKBONE
- Vector backbonepUC19(Search Vector Database)
- Vector typeBacterial Expression, CRISPR
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Top10
- Copy numberHigh Copy
GENE/INSERT
- Gene/Insert nameguide RNA (bacteria)
- Insert Size (bp)102
PromoterBBa_J23119 (SpeI)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteSpeI (not destroyed)
3′ cloning siteHindIII (not destroyed)
5′ sequencing primern/a
3′ sequencing primern/a
SEQUENCE INFORMATION
>Contains bacteria guide RNA expression
gAATTCTAAAGATCTTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTAACTTTCAGTTTAGCGGTCT
GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGT
CGGTGCTTTTTTTGAAGCTTGGGCCCGAACAAAAACTCatctcagaagaggatctgaatagcgccgtcga
ccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattt
tcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtag
cgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtgggg
tctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactGGGCC
TTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGgcttcctcgctc
actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt
tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg
taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgc
tcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg
tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc
gctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg
cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa
gacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgc
tacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctg
ctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg
gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt
ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa
aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaa
cttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatc
catagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct
gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg
ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctag
agtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc
tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgt
tgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatc
actcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgact
ggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaa
tacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcg
aaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatct
tcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagg
gaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatca
gggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgc
acatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaata
ggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctg
For your References section:
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860
BioVector NTCC保藏中心提供
- 公告/新聞
