pfu高保真聚合酶高表達質粒載體菌種-BioVector NTCC保藏中心
- 價 格：￥6920
- 貨 號：pfu DNA polymerase expression plasmid
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
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pfu DNA polymerase聚合酶高表達質粒載體菌種
DescriptionBack to Top
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific? Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5′→ 3′ polymerase activity, 3′→ 5′ exonuclease activity and will generate blunt-ended products.Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.
Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| DMSO | 100% |
Advantages and FeaturesBack to Top
Features
Robust Reactions - Maximal success with minimal optimization.
Extreme Fidelity - > 50x greater than Taq
High Speed - Dramatically reduced extension times (10x faster than Pfu)
High Yield - Increased product yield using minimal amount of enzyme.
Versatile - Can be used for routine per as well as long or difficult templates.
Applications
PCR
Cloning
Long or Difficult Amplification
High-Throughput PCR
Properties and UsageBack to Top
Storage Temperature
-20°CHeat Inactivation
NoUnit Assay Conditions
25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM b-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.NotesBack to Top
Products specifications for individual components in the Phusion High-Fidelity PCR Master Mix with HF Buffer are available separately.
ReferencesBack to Top
Frey, B. and Suppman, B. (1995). Biochemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.
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