AFRC MAC 13雜交瘤細(xì)胞Hybridoma cells BioVector NTCC保藏中心
Reactibity反應(yīng)性:Anti- glycoprotein of Chlamydomonas reinhardii
Antibody Isotype抗體類性:IgM

【Organism物種來源】Mouse
【Tissue組織來源】
【Cell Type: 細(xì)胞類型】 Hybridoma
【Product Format產(chǎn)品狀態(tài)】 frozen/live culture
【Morphology細(xì)胞形態(tài)】
【Culture Properties細(xì)胞特性】
【Biosafety Level生物安全級(jí)別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Myeloma:
Fusion Species: Mouse x Mouse Hybridoma
【Immunogen:】
【Cell Line Description:】
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】
【Images細(xì)胞圖片】 Cell Micrograph
【Derivation衍生細(xì)胞】 -
【Clinical Data臨床數(shù)據(jù)】
【Comments其他描述】
【Complete Growth Medium完全培養(yǎng)基】DMEM + 2mM glutamine + 20% Foetal Bovine Serum (FBS)
【Subculturing傳代方法】
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability. Establishment of an actively proliferating culture may take up to 2 weeks. On resuscitation a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of prewarmed growth media+. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10? cells/ml. Place culture flask flat and observe regularly until viable proliferating cells are seen. Often hybridoma cultures may benefit from being resuspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation. Once the culture is established in culture the FBS can be reduced to 10%.
【Cryopreservation凍存條件】
【Freeze Medium凍存培養(yǎng)基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養(yǎng)條件】
【Atmosphere培養(yǎng)環(huán)境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養(yǎng)溫度】 37°C
【STR Profile圖譜】
【Population Doubling Time倍增殖時(shí)間】
【References參考文獻(xiàn)】

【Supplier細(xì)胞供應(yīng)廠家】BioVector NTCC Inc.
TEL:+86-010-53513060
【W(wǎng)ebsite網(wǎng)址】http://www.biovector.net