質粒名稱:mVenus-C1

目錄編號:54651

存儲實驗室:Michael Davidson

用途:C1克隆載體載體骨架基本信息載體名稱:mVenus-C1載體抗性:卡那霉素空載體大小:4750 bp載體類型:哺乳細胞表達篩選標記:新霉素（G418）高拷貝/低拷貝:高拷貝生長條件:37 ℃插入基因信息插入基因名稱:None標簽/融合蛋白:mVenus（N端）文獻引用方法材料和方法部分:mVenus-C1 was a gift from Michael Davidson (Addgene plasmid # 54651)載體質粒圖譜和多克隆位點信息mVenus-C1

載體圖譜

載體簡介

載體序列LOCUS       mVenus-C1 Sequencing Result851 bp DNASYNDEFINITION  mVenus-C1 Sequencing ResultACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..851                     /organism="mVenus-C1 Sequencing Result"                     /mol_type="other DNA"     CDS             39..836                     /label="ORF frame 3"     gene            42..776                     /label="EYFP (variant)"                     /gene="EYFP (variant)"     misc_feature    complement(84..105)                     /label="EGFP_N_primer"     misc_feature    492..575                     /label="M153T,V163A,S175G (Venus)"     misc_feature    692..713                     /label="EGFP_C_primer"ORIGIN    1 GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC   61 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT  121 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA  181 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG  241 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG  301 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA  361 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG  421 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA  481 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA  541 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC  601 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC  661 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG  721 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGCTCAAG  781 CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA  841 TCATAATCAG C//