pCDH-EF1-Luc2-P2A-copGFP BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心 PURPOSE Express Luc2 and copGFP
Vector backbone pCDH-CMV-MCS (Search Vector Database) Backbone manufacturer Systembio Backbone size w/o insert (bp) 7133 Modifications to backbone ClaI/SalI fragment was replaced with EF1 + new MCS Vector type Mammalian Expression, Lentiviral GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) NEB Stable Copy number High Copy GENE/INSERT Gene/Insert name Luc2-P2A-copGFP Species Synthetic Insert Size (bp) 2498 Promoter EF-1 CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site BamHI (not destroyed) 3′ cloning site EcoRI (unknown if destroyed) 5′ sequencing primer GCACTTGATGTAATTCTCC 3′ sequencing primer CAACACCACGGAATTGTCAG
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