pLX302 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心                      

Vector backbone pLKO
(Search Vector Database)
Backbone size (bp) 9573
Vector type Mammalian Expression, Lentiviral ; Gateway Destination vector
Selectable markers Puromycin
GROWTH IN BACTERIA

Bacterial Resistance(s) Chloramphenicol and Ampicillin
Growth Temperature 30°C
Growth Strain(s) ccdB Survival
Growth instructions ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
Copy number Low Copy
GENE/INSERT

Gene/Insert name None
Tag / Fusion Protein
V5 (C terminal on backbone)
CLONING INFORMATION

Cloning method Gateway Cloning
5′ sequencing primer CMV-F
3′ sequencing primer WPRE-R
There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
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