pCFJ104 - Pmyo-3::mCherry::unc-54線蟲紅色熒光表達載體

咽部表達，帶有myo-3 promoter啟動子. unc-54 utr. Amp抗性。<br/>Red mCherry expression in the pharynx driven by the myo-3promoter. unc-54 utr.<br/>Vector backbone pDESTR4-R3

(Search Vector Database)

Backbone manufacturer Invitrogen

Backbone size w/o insert (bp) 2416

Vector type Worm Expression

GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin

Growth Temperature 37°C

Growth Strain(s) DH5alpha

Copy number High Copy

GENE/INSERT

Gene/Insert name Pmyo-3::mCherry

Species C. elegans (nematode)

Insert Size (bp) 4224

Tag / Fusion Protein

mCherry

CLONING INFORMATION

Cloning method Gateway Cloning

5′ cloning site attR4(destroyed during cloning)

3′ cloning site attR3(destroyed during cloning)

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