Chromobacterium violaceum CV026群體感應報告菌株AHL生物感應器 BioVector NTCC質粒載體菌種細胞基因保藏中心 Chromobacterium violaceum CV026
Cat#: NTCC503714

Current Name: Chromobacterium violaceum
Other Collection No: CV026
Type Strain: No
Family: Neisseriaceae
Accession Date: 14/06/2007
Taxonomy: TaxLink: S793 (Chromobacterium violaceum bergonzini 1880) - Date of change: 31/05/2007
Other: biosensor strain
Description: Double mini-Tn5 mutant derived from ATCC 31532, KanR, HgR, cvil::Tn5 xylE, plus spontaneous StrR. AHL biosensor, produces violacein pigment only in the presence of exogenous AHL.

Chromobacterium violaceum (C. violaceum), a Gram negative bacterium having a wide geographic distribution, produces the pigment violacein in response to QS regulated gene expression. Further, the whole genome studies on C. violaceum showed that violacein production is regulated by vioD, vioC, vioB and vioA genes arranged in an operon through a QS system mediated by AHL. A biosensor strain, C. violaceum CV026 (mini-Tn5 mutant of wild type strain), deficient in autoinducer synthase which requires exogenous addition of AHL to produce violacein was developed for studying various QS mechanisms, and it offers a convenient tool for the biological assay of screening QS inhibitors.
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