Agrobacterium tumefaciens NTL4群體感應信號報告菌株 BioVector NTCC質粒載體菌種細胞基因保藏中心
- 價 格：￥27935
- 貨 號：Agrobacterium tumefaciens NTL4群體感應信號報告菌株
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
Agrobacterium tumefaciens NTL4群體感應信號報告菌株 BioVector NTCC質粒載體菌種細胞基因保藏中心 Agrobacterium tumefaciens NTL4
Cat#:NTCC596666
Deposited As Agrobacterium tumefaciens
Strain Designations NTL4(pZLR4)
Application Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level 1
Type Strain no
Comments The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium LB medium with 15 mcg/ml gentamycin
Growth Conditions Temperature: 28°C
Atmosphere: Aerobic
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
References Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net [Supplier來源] http://www.biovector.net
Cat#:NTCC596666
Deposited As Agrobacterium tumefaciens
Strain Designations NTL4(pZLR4)
Application Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level 1
Type Strain no
Comments The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium LB medium with 15 mcg/ml gentamycin
Growth Conditions Temperature: 28°C
Atmosphere: Aerobic
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
References Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net [Supplier來源] http://www.biovector.net
- 公告/新聞
