pAG31酵母Bialaphos抗性篩選標記載體 BioVector NTCC質粒載體菌種細胞基因保藏中心                      Vector backbone pFA6
(Search Vector Database)
Modifications to backbone Transformed E.coli cells are resistant to ampicillin. The plasmid contains the pat1 gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyl-transferase and confers resistance to the antibiotic bialaphos of transformed yeasts. Other plasmids conferring bialaphos resistance: pAG29. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. The plasmid provides the possibility for a marker rescue by homologous recombination at the direct repeats.
Vector type Yeast replicating
Selectable markers Bialaphos
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name patMX3
Species Streptomyces viridochromogenes
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
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