痘病毒載體Attenuated modified vaccinia Ankara (MVA)strain of vaccinia virus BioVector NTCC質粒載體菌種細胞基因保藏中心
Modified vaccinia virus Ankura (MVA), a highly attenuated strain of vaccinia virus (VV) that is unable to replicate in most mammalian cells, was evaluated as an expression vector for a model tumor associated antigen (TAA) and as a potential anti-cancer vaccine. We employed an experimental murine model in which an adenocarcinoma tumor line, CT26.CL25, was stably transfected with a model TAA, β-galactosidase (β-gal). Mice injected intrumuscularly with a recombinant MVA (rMVA) expressing β-gal (MVA-LZ), were protected from a lethal intravenous (i. v.) challenge with CT26.CL25. In addition, splenocytes from mice primed with MVA-LZ were therapeutically effective upon adoptive transfer to mice bearing pulmonary metastases of the CT26.CL25 tumor established 3 days earlier. Most importantly, i.v. inoculation with MVA-LZ resulted in significantly prolonged survival of mice bearing three day old pulmonary metastases. This prolonged survival compared favorably to mice treated with a replication competent recombinunt VV expressing β-gal. These findings indicate that rMVA is an efficacious alternative to the more commonly used replication competent VVfor the development of new recombinant anti-cancer vaccines.
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