兔角膜上皮細胞SIRC BioVector NTCC質粒載體菌種細胞基因保藏中心
Cell Line Name: SIRC


Keywords: Rabbit Cornea
Cell Line Description: Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Species: Rabbit
Tissue of Origin: Cornea

CellType: Fibroblast

Growth Mode: Adherent
Karyotype: 2n = 44


Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37°C.
Culture Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Originator: No
Country: USA
References: Virus susceptibility: Science 1965;149:633; Proc Soc Exp Med 1966;122:783; Proc Soc Exp Med 1967;125:12
Additional Bibliography: Niederkorn JY1, Meyer DR, Ubelaker JE, Martin JH.,Ultrastructural and immunohistological characterization of the SIRC corneal cell line. In Vitro Cell Dev Biol. 1990 Sep;26(9):923-30. PMID:2228906

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