pBEN276革蘭氏陰性菌Tn7轉座子Luc熒光表達載體 BioVector NTCC質粒載體菌種細胞基因保藏中心
pBEN276革蘭氏陰性菌Tn7轉座子Lux熒光表達載體，帶有luxCDABE熒光報告基因，Tn轉座子元件
Intergration of luciferase operon in Tn7 insertion site of Gram negative bacteria
Vector backbone pGRG25
(Search Vector Database)
Backbone manufacturer Nancy Craig - Johns Hopkins University (Addgene BioVector-016665)
Backbone size w/o insert (bp) 12500
Total vector size (bp) 19671
Vector type Luciferase
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 30°C
Growth Strain(s) DH5alpha
Copy number Low Copy
GENE/INSERT

Gene/Insert name luxCDABE
Species Photorhabdus luminescens
Insert Size (bp) 6200
GenBank IDAF403784
Promoter frr
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site XhoI (not destroyed)
3′ cloning site NotI (not destroyed)
5′ sequencing primer AATTGCCCGTCGTATTAAAG
3′ sequencing primer GTAGCGTCGTAAGCTAATAC
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net
【Supplier來源】BioVector NTCC Inc.
【Website網址】 http://www.biovector.net