• pUT-miniTn5 Cm

• 
  

• 圖譜Map

• 

  

  

  

  
  

The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

Advantages:

• Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.

• Wide range of inserted fragment sizes.

• Possibility of multiple insertions in the same strain.

• Easy modification of the cloning vector.

• Use of cleavage sites of the rare cutters NotI and SfiI.

• Wide range of Gram negative recipient strains.

Some of the most common applications of pUTmini-Tn5 vectors are:

• Cloning.

• Insertional mutagenesis.

• Promoter probing.

• Generation of conditional phenotypes and non-polar mutations.

• Analysis of transcriptional terminators.

• Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.

• 
  

• ---------------------

• pUT mini-Tn5 Cm kit

  • pUT mini-Tn5 Cm, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Km kit

  • pUT mini-Tn5 Km, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Sm/Sp kit

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Tc kit

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  PANC-1 mini-Tn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  
  

  PANC-1plus miniTn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn5Tel kit

  • pUT mini-Tn5Tel vector, 2 μg.

  • Potassium Tellurite, 100mg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

另有

質(zhì)粒載體菌株細(xì)胞株基因保藏中心-Biovector Science Lab,Inc.可提供數(shù)萬種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純化、病毒包裝、基因沉默RNAi等服務(wù)。作為美國質(zhì)粒載體菌株細(xì)胞株基因保藏中心分庫及唯一辦事處，Biovector Inc.可為您提供便捷一站式的產(chǎn)品進(jìn)口服務(wù)，到貨快捷，步驟簡便。通過Google搜索"Biovector+您所需資源名稱",您一定能找到所需的資源和技術(shù)服務(wù)。如有需要請聯(lián)系：

Dr Xu,Senior Scientist
電郵Biovector@163.com
電話18901268599

訂購合同下載請點(diǎn)擊：Biovector質(zhì)粒載體菌株細(xì)胞基因訂購合同-Biovector Science Lab

^_^尋找您所需質(zhì)粒載體菌株基因細(xì)胞株等資源的竅門：在Google，Baidu等搜索引擎中搜索“Biovector您所需的資源名稱”結(jié)果即刻閃現(xiàn)，馬上試試吧^_^

點(diǎn)擊瀏覽器菜單“編輯”-->"查找"或者直接按“Ctrl+F”查找您所需資源

由于頁面字?jǐn)?shù)限制，尚有很多質(zhì)粒載體菌株基因片段名稱未列出，如查找不到您所需資源，請發(fā)郵件或電話查詢。

Biovector,Inc質(zhì)粒載體菌株細(xì)胞株基因保藏中心列表網(wǎng)址http://biovector.blog.163.com

原核表達(dá)載體

• 真核表達(dá)載體

• 哺乳動物表達(dá)載體

• 熒光定位表達(dá)載體

• 慢病毒/腺病毒/逆病毒..

• RNAi基因沉默載體

• 乳酸菌表達(dá)載體/乳酸桿..

• 枯草芽孢桿菌表達(dá)載體

• 釀酒/畢赤酵母表達(dá)載體..

• 工具酶質(zhì)粒Taq,KO..

• GS篩選表達(dá)系統(tǒng)

• 疫苗表達(dá)載體/抗體表達(dá)

• 革蘭氏陽性菌表達(dá)/金葡..

• 谷氨酸棒狀桿菌表達(dá)載體

• 噬菌體展示系統(tǒng)/抗體表..

• 酵母單雜交/雙雜交/三..

• BiFC雙分子熒光載體..

• 昆蟲/桿狀病毒表達(dá)載體

• 轉(zhuǎn)座子質(zhì)粒載體/Pig..

• 基因重組/敲除載體/基..

• Gateway系統(tǒng)載體

• 植物表達(dá)載體/熒光定位..

• 農(nóng)桿菌感受態(tài)細(xì)胞及菌株

• 信號通路報(bào)告載體

• 四環(huán)素誘導(dǎo)表達(dá)系統(tǒng)

• 真菌表達(dá)/黑曲霉/黃曲..

• 病毒包裝系統(tǒng)

• 各種大腸桿菌及感受態(tài)細(xì)..

• Addgene質(zhì)粒/A..

• 人鼠基因庫cDNA,O..

• 細(xì)胞株

• 鏈霉菌表達(dá)/敲除/鏈球..

• 細(xì)胞永生化載體

• iPS干細(xì)胞重編程載體

• 多基因表達(dá)/雙啟動子載..

• 質(zhì)粒構(gòu)建技術(shù)服務(wù)

• 基因克隆技術(shù)服務(wù)

• 果蠅細(xì)胞表達(dá)載體

• 多宿主載體/假單胞菌表..

• 懸浮細(xì)胞大規(guī)模表達(dá)載體..

• 報(bào)告基因載體

• 逆轉(zhuǎn)錄病毒載體

• 腺病毒載體/腺相關(guān)病毒..

• LC3自噬質(zhì)粒載體

• 線蟲表達(dá)載體RNAi

• 哺乳動物雙雜交系統(tǒng)

• 蛻皮激素誘導(dǎo)表達(dá)系統(tǒng)

• **質(zhì)粒載體

• 原核熒光表達(dá)載體

• 溫度敏感型表達(dá)載體

• 共表達(dá)載體

• Cre/LoxP載體C..