LRG (Lenti_sgRNA_EFS_GFP) BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
LRG (Lenti_sgRNA_EFS_GFP)慢病毒sgRNA組成型表達(dá)質(zhì)粒熒光報告質(zhì)粒，hU6啟動子+sgRNA，EFS1啟動子+GFP綠色熒光報告基因。Amp抗性。
Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.
Vector backbone Lenti virus
(Search Vector Database)
Backbone manufacturer Modified from lentiCRISPRv1 (Addgene Plasmid 49535)
Backbone size (bp) 9294
Vector type Mammalian Expression, Lentiviral, CRISPR
Promoter U6 promoter-driven sgRNA expression and EFS promoter-driven GFP expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number High Copy
CLONING INFORMATION

Cloning method Ligation Independent Cloning
5′ sequencing primer AACCGGTGCCTAGAGAAGGT
(Common Sequencing Primers)
RESOURCE INFORMATION

A portion of this plasmid was derived from a plasmid made by The U6-sgRNA-EFS-GFP vector was derived from the lentiCRISPR-Cas9 plasmid (Addgene: # 49535) by removing the hCas9 cDNA and replacing the Puro cassette with GFP reporter.
BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心電話：+86-010-53513060網(wǎng)址：www.biovector.net
【Supplier來源】BioVector NTCC Inc.
【W(wǎng)ebsite網(wǎng)址】 http://www.biovector.net