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gRNA-trp-HYBYeast ExpressionJingBlock product of trp1 deletion gRNA cassette (Synthetic)Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.?Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
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