pCDNA3.1-3×HA-N細胞表達質粒載體

pCDNA3.1-3×HA-N細胞過表達載體，MCS區目的基因N端帶有3xHA標簽，Amp抗性，G418細胞篩選標記。
啟動子: CMV promoter
復制子: pUC ori，F1 ori
終止子: BGH poly（A）signal
質粒分類: 哺乳系列質粒；哺乳表達質粒；pCDNA系列質粒
質粒大小: 5541bp
質粒標簽: N-3×HA
原核抗性: 氨芐青霉素Amp（100μg/ml）
篩選標記: 新霉素Neo/G418
克隆菌株: DH5α等大腸桿菌
培養條件: 37℃，有氧，LB
表達宿主: 293T等哺乳細胞
培養條件: 37℃，5%CO2
誘導方式: 無需誘導
5'測序引物: pCDNA3.1-F（CTAGAGAACCCACTGCTTAC）
3'測序引物: pCDNA3.1-R（TAGAAGGCACAGTCGAGG）
質粒簡介
      pCDNA3.1-3×HA-N質粒是一種高效表達的哺乳質粒。該質粒含有Amp抗性，可轉至DH5α中轉化。培養條件為LB培養基，37℃。
      pCDNA3.1-3×HA-N vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site.High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Neomycin resistance gene for selection of stable cell lines. There are three HA tags on the N side.Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
質粒圖譜

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net