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pGL4.73[hRluc /SV40]熒光報告質粒
別名: pGL4.73
啟動子: SV40
復制子: pUC ori,SV40 ori
終止子: SV40 poly(A) signal
質粒分類: 哺乳細胞,信號通路報告載體
質粒大小: 3921bp
原核抗性: 氨芐青霉素Amp
克隆菌株: 大腸桿菌DH5α
培養條件: 37℃,有氧,LB
表達宿主: 哺乳細胞
誘導方式: 無須誘導,瞬時表達
5'測序引物: RVP3:CTAGCAAAATAGGCTGTCCC
3'測序引物: 根據序列設計引物
質粒簡介
The pGL4.73[hRluc/SV40] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.
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