- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
pEZ BAC載體
Map圖譜
CopyRight? v2.0 BAC Cloning Kits (pSMART? BAC and pEZ? BAC)
Efficiently clone any size insert up to 200kb.
Create libraries from A/T- or G/C-rich genomes.
Clone gene clusters or operons.
High insert stability.
Inducible copy number.
Ordering
Resources
Each CopyRight? v2.0 BAC Cloning Kit contains: pre-cut vector (pSMART BAC with a choice of BamHI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, Arabinose Induction Solution, and complete protocols. Each CopyRight pEZ BAC Cloning Kit contains: pre-cut CopyRight vector (pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect? 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>2 × 1010 cfu/μg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.
Please note that the following vectors have been discontinued: pSMART BAC EcoRI (catalog numbers 42031-1, 42031-2) and pEZ BAC BamHI (catalog numbers 42007-1, 42007-2 and 42012-1).”
| Document | File Name | Type |
| Manual | MA028-CopyRight-v2.0-BAC-Cloning-Kits | |
| MSDS | MSDS.pdf | |
| Product Flyer | BigEasy-US-2013 | |
| Citations | CopyRight? v2.0 BAC Cloning Kits (pSMART? BAC and pEZ? BAC) - Citations | LINK |
| Vector Sequence | Sequence(s) | Link |
Cloning vector pEZ BAC, complete sequence
AAGGAATATTCAGCAATTTGCCCGTGCCGAAGAAAGGCCCACCCGTGAAG
GTGAGCCAGTGAGTTGATTGCTACGTAAATAACTTCGTATAGCATACATT
ATACGAAGTTATGGACTAGGCGCGCCAGAAGAGAGAAAGAAGGAAAGCGG
CCGCACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAA
TGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTC
CGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGA
AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGAGACTATAGAAT
ACTCAAGCTTGCATGCGATACGTATCGTTAACGATGGATCCGACGCACGT
GCGAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT
ACAACGTCGTGACTGGGAAAACCCTGGCGTCACCCAACTTAATCGCCTTG
CAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACC
GATCGCCCTTCCCAACAGTTGCGCAGCTGAATGGCGAATGGCGCCTGATG
CGGTATTTTCTCCTTACGGCGGCCGCTTGACATAACTTCGTATAGCATAC
ATTATACGAAGTTATGTTTAAACATTAGCAGAAAGTCAAAGGCCTCCGGT
CGGAGGCTTTTGACTAAAACTTCCCTTGGGGTTATCATTGGGCCGAGACC
GCCTGAAGAGGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGG
AAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTC
TTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC
GGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGG
TCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA
ATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGT
CAAATAATCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACT
TAACGTAAAAGCAACTTCAGACAATACAAATCAGCGACACTGAATACGGG
GCAACCTCATGTCGCCTGAAGAGTGAGACCGGCCCTGATCGGCACGTAAG
AGGCTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTT
TTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAA
ATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACA
TTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTC
AGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAG
TTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC
GGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTG
TTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCG
CTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTC
GCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGT
TTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACC
AGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTT
CACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG
CGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATG
CTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAA
ATGTAATCACCTGGCTCACCTTCGGGTGGGCCTTTCACACTTGCATCGGA
TGCAGCCCGGTGAACGTGCCGGCACGGCCTGGGTAACCAGGTATTTTGTC
CACATAACCGTGCGCAAAATGTTGTGGATAAGCAGGACACAGCAGCAATC
CACAGCAGGCATACAACCGCACACCGAGGTTACTCCGTTCTACAGGTTAC
GACGACATGTCAATACTTGCCCTTGACAGGCATTGATGGAATCGTAGTCT
CACGCTGATAGTCTGATCGACAATACAAGTGGGACCGTGGTCCCAGACCG
ATAATCAGACCGACAACACGAGTGGGATCGTGGTCCCAGACTAATAATCA
GACCGACGATACGAGTGGGACCGTGGTCCCAGACTAATAATCAGACCGAC
GATACGAGTGGGACCGTGGTTCCAGACTAATAATCAGACCGACGATACGA
GTGGGACCGTGGTCCCAGACTAATAATCAGACCGACGATACGAGTGGGAC
CATGGTCCCAGACTAATAATCAGACCGACGATACGAGTGGGACCGTGGTC
CCAGTCTGATTATCAGACCGACGATACGAGTGGGACCGTGGTCCCAGACT
AATAATCAGACCGACGATACGAGTGGGACCGTGGTCCCAGACTAATAATC
AGACCGACGATACGAGTGGGACCGTGGTCCCAGTCTGATTATCAGACCGA
CGATACAAGTGGAACAGTGGGCCCAGAGAGAATATTCAGGCCAGTTATGC
TTTCTGGCCTGTAACAAAGGACATTAAGTAAAGACAGATAAACGTAGACT
AAAACGTGGTCGCATCAGGGTGCTGGCTTTTCAAGTTCCTTAAGAATGGC
CTCAATTTTCTCTATACACTCAGTTGGAACACGAGACCTGTCCAGGTTAA
GCACCATTTTATCGCCCTTATACAATACTGTCGCTCCAGGAGCAAACTGA
TGTCGTGAGCTTAAACTAGTTCTTGATGCAGATGACGTTTTAAGCACAGA
AGTTAAAAGAGTGATAACTTCTTCAGCTTCAAATATCACCCCAGCTTTTT
TCTGCTCATGAAGGTTAGATGCCTGCTGCTTAAGTAATTCCTCTTTATCT
GTAAAGGCTTTTTGAAGTGCATCACCTGACCGGGCAGATAGTTCACCGGG
GTGAGAAAAAAGAGCAACAACTGATTTAGGCAATTTGGCGGTGTTGATAC
AGCGGGTAATAATCTTACGTGAAATATTTTCCGCATCAGCCAGCGCAGAA
ATATTTCCAGCAAATTCATTCTGCAATCGGCTTGCATAACGCTGACCACG
TTCATAAGCACTTGTTGGGCGATAATCGTTACCCAATCTGGATAATGCAG
CCATCTGCTCATCATCCAGCTCGCCAACCAGAACACGATAATCACTTTCG
GTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGAC
ACCAGATACTCTTCGACCGAACGCCGGTGTCTGTTGACCAGTCAGTAGAA
AAGAAGGGATGAGATCATCCAGTGCGTCCTCAGTAAGCAGCTCCTGGTCA
CGTTCATTACCTGACCATACCCGAGAGGTCTTCTCAACACTATCACCCCG
GAGCACTTCAAGAGTAAACTTCACATCCCGACCACATACAGGCAAAGTAA
TGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAATCCACC
ATCGGGGCAGCTGGTGTCGATAACGAAGTATCTTCAACCGGTTGAGTATT
GAGCGTATGTTTTGGAATAACAGGCGCACGCTTCATTATCTAATCTCCCA
GCGTGGTTTAATCAGACGATCGAAAATTTCATTGCAGACAGGTTCCCAAA
TAGAAAGAGCATTTCTCCAGGCACCAGTTGAAGAGCGTTGATCAATGGCC
TGTTCAAAAACAGTTCTCATCCGGATCTGACCTTTACCAACTTCATCCGT
TTCACGTACAACATTTTTTAGAACCATGCTTCCCCAGGCATCCCGAATTT
GCTCCTCCATCCACGGGGACTGAGAGCCATTACTATTGCTGTATTTGGTA
AGCAAAATACGTACATCAGGCTCGAACCCTTTAAGATCAACGTTCTTGAG
CAGATCACGAAGCATATCGAAAAACTGCAGTGCGGAGGTGTAGTCAAACA
ACTCAGCAGGCGTGGGAACAATCAGCACATCAGCAGCACATACGACATTA
ATCGTGCCGATACCCAGGTTAGGCGCGCTGTCAATAACTATGACATCATA
GTCATGAGCAACAGTTTCAATGGCCAGTCGGAGCATCAGGTGTGGATCGG
TGGGCAGTTTACCTTCATCAAATTTGCCCATTAACTCAGTTTCAATACGG
TGCAGAGCCAGACAGGAAGGAATAATGTCAAGCCCCGGCCAGCAAGTGGG
CTTTATTGCATAAGTGACATCGTCCTTTTCCCCAAGATAGAAAGGCAGGA
GAGTGTCTTCTGCATGAATATGAAGATCTGGTACCCATCCGTGATACATT
GAGGCTGTTCCCTGGGGGTCGTTACCTTCCACGAGCAAAACACGTAGCCC
CTTCAGAGCCAGATCCTGAGCAAGATGAACAGAAACTGAGGTTTTGTAAA
CGCCACCTTTATGGGCAGCAACCCCGATCACCGGTGGAAATACGTCTTCA
GCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTC
AATTGTATAACCAACACGTTGCTCAACCCGTCCTCGAATTTCCATATCCG
GGTGCGGTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAA
ACCCCAACTAAATCCGCTGCTTCACCTATTCTCCAGCGCCGGGTTATTTT
CCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAATGGCGATAGCCTTCG
TCATTTCATGACCAGCGTTTATGCACTGGTTAAGTGTTTCCATGAGTTTC
ATTCTGAACATCCTTTAATCATTGCTTTGCGTTTTTTTATTAAATCTTGC
AATTTACTGCAAAGCAACAACAAAATCGCAAAGTCATCAAAAAACCGCAA
AGTTGTTTAAAATAAGAGCAACACTACAAAAGGAGATAAGAAGAGCACAT
ACCTCAGTCACTTATTATCACTAGCGCTCGCCGCAGCCGTGTAACCGAGC
ATAGCGAGCGAACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAG
CTCTTACGCTCAGCGCAAGAAGAAATATCCACCGTGGGAAAAACTCCAGG
TAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTGTGATAATC
AACCCTCATCAATGATGACGAACTAACCCCCGATATCAAGTCACATGACG
AAGGGAAAGAGAAGGAAATCAACTGTGACAAACTGCCCTCAAATTTGGCT
TCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTGAA
GGAAACAGCAAAACTGTGACAAATTACCCTCAGTAGGTCAGAACAAATGT
GACGAACCACCCTCAAATCTGTGACAGATAACCCTCAGACTATCCTGTCG
TCATGGAAGTGATATCGCGGAAGGAAAATACGATATGAGTCGTCTGGCGG
CCTTTCTTTTTCTCAATGTATGAGAGGCGCATTGGAGTTCTGCTGTTGAT
CTCATTAACACAGACCTGCAGGAAGCGGCGGCGGAAGTCAGGCATACGCT
GGTAACTTTGAGGCAGCTGGTAACGCTCTATGATCCAGTCGATTTTCAGA
GAGACGATGCCTGAGCCATCCGGCTTACGATACTGACACAGGGATTCGTA
TAAACGCATGGCATACGGATTGGTGATTTCTTTTGTTTCACTAAGCCGAA
ACTGCGTAAACCGGTTCTGTAACCCGATAAAGAAGGGAATGAGATATGGG
TTGATATGTACACTGTAAAGCCCTCTGGATGGACTGTGCGCACGTTTGAT
AAACCAAGGAAAAGATTCATAGCCTTTTTCATCGCCGGCATCCTCTTCAG
GGCGATAAAAAACCACTTCCTTCCCCGCGAAACTCTTCAATGCCTGCCGT
ATATCCTTACTGGCTTCCGCAGAGGTCAATCCGAATATTTCAGCATATTT
AGCAACATGGATCTCGCAGATACCGTCATGTTCCTGTAGGGTGCCATCAG
ATTTTCTGATCTGGTCAACGAACAGATACAGCATACGTTTTTGATCCCGG
GAGAGACTATATGCCGCCTCAGTGAGGTCGTTTGACTGGACGATTCGCGG
GCTATTTTTACGTTTCTTGTGATTGATAACCGCTGTTTCCGCCATGACAG
ATCCATGTGAAGTGTGACAAGTTTTTAGATTGTCACACTAAATAAAAAAG
AGTCAATAAGCAGGGATAACTTTGTGAAAAAACAGCTTCTTCTGAGGGCA
ATTTGTCACAGGGTTAAGGGCAATTTGTCACAGACAGGACTGTCATTTGA
GGGTGATTTGTCACACTGAAAGGGCAATTTGTCACAACACCTTCTCTAGA
ACCAGCATGGATAAAGGCCTACAAGGCGCTCTAAAAAAGAAGATCTAAAA
ACTATAAAAAAAATAATTATAAAAATATCCCCGTGGATAAGTGGATAACC
CCAAGGGAAGTTTTTTCAGGCATCGTGTGTAAGCAGAATATATAAGTGCT
GTTCCCTGGTGCTTCCTCGCTCACTCGACCGGGAGGGTTCGAGAAGGGGG
GGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACAGGGCGCAGCCCTGGT
TAAAAACAAGGTTTATAAATATTGGTTTAAAAGCAGGTTAAAAGACAGGT
TAGCGGTGGCCGAAAAACGGGCGGAAACCCTTGCAAATGCTGGATTTTCT
GCCTGTGGACAGCCCCTCAAATGTCAATAGGTGCGCCCCTCATCTGTCAG
CACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCTCATCTGTCAGTAGTC
GCGCCCCTCAAGTGTCAATACCGCAGGGCACTTATCCCCAGGCTTGTCCA
CATCATCTGTGGGAAACTCGCGTAAAATCAGGCGTTTTCGCCGATTTGCG
AGGCTGGCCAGCTCCACGTCGCCGGCCGAAATCGAGCCTGCCCCTCATCT
GTCAACGCCGCGCCGGGTGAGTCGGCCCCTCAAGTGTCAACGTCCGCCCC
TCATCTGTCAGTGAGGGCCAAGTTTTCCGCGAGGTATCCACAACGCCGGC
GGCCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGCGCGTT
TGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGG
TGAGCGTCGGA
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