pcDNA3 GFP LIC cloning vector (6D)哺乳綠色熒光表達質粒
This is an empty LIC vector derived from pcDNA3. It adds a GFP gene to the C-terminus of your protein of interest.

GFP has a excitation max of 489 nm and an emission max of 510 nm.

To clone into this vector, add the following tags to your PCR primers:

LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)

LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC

Do NOT include a stop codon with your reverse primer.

T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.

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