pCMVbeta
Description
High copy number eukaryotic vector, pCMVβ contains the full-length lacZβ-galactosidase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species where constitutive expression is desired.

The β-galactosidase enzyme expression is enhanced by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40 late polyadenylylation signal. pCMVβ expression vector also contains β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. pCMVβ vector has been tested to generate up to 2530u/mg cell extract (MacGregor, and Caskey). In addition, the β-galactosidase gene can be excised using the NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pSV40β vector system,
Alt Name: pCMV-beta-gal, pCMV-LacZ
Analyze: Sequence
Plasmid Type: Mammalian Expression
Promotor: CMV
Cloning Method: Unknown
Size: 7164
5' Sequencing 1 Primer: CMV-F
3' Sequencing 1 Primer: EBV-rev
Bacterial Resistance: Ampicillin
Notes:
ATCC size is 7300 bp. The order of the major features in this plasmid is [1]: pUC19 - EcoRI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals -NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, monkey COS cells, vertebrate cells, E.coli. Related vectors: pADbeta, pNASSbeta, pSVbeta, pTKbeta.

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