MG15-luc (mut p53 binding sites)?
- 價 格:¥9852
- 貨 號:MG15-luc (mut p53 binding sites)?
- 產 地:北京
- BioVector NTCC典型培養物保藏中心
- 聯系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作QQ:1843439339 (微信同號)
郵件:Biovector@163.com
手機:18901268599
地址:北京
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MG15-luc (mut p53 binding sites)?
MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話:+86-010-53513060
網址:www.biovector.net
MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話:+86-010-53513060
網址:www.biovector.net
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