pEYFP-Mem細胞膜定位黃色熒光表達載體

質粒簡介

   pEYFP-Mem encodes a fusion protein consisting of the N-terminal 20 aminoacids of neuromodulin,also called GAP-43, and a yellow-green fluorescentvariant of the enhanced green fluorescent protein (EGFP). The neuromodulinfragment contains a signal for posttranslational palmitoylation of cysteines 3and 4 that targets EYFP to membranes. The EYFP gene contains four amino acidsubstitutions previously published as GFP-10C. The fluorescence excitationmaximum of EYFP is 513 nm, and the emission maximum is 527 nm (in theyellow-green region).

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