pCEP4/CAT
啟動子: CMV
復制子: pUC ori
終止子: SV40 poly(A) signal
質(zhì)粒分類: 哺乳細胞，蛋白過表達載體
質(zhì)粒大小: 10891bp
原核抗性: Amp
篩選標記: Hyg
克隆菌株: DH5α
培養(yǎng)條件: 37℃，有氧，LB
表達宿主: 哺乳細胞
誘導方式: 無須誘導，瞬時表達
5'測序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'測序引物: Sv40-polyA-R：GAAATTTGTGATGCTATTGC
質(zhì)粒簡介
pCEP4 is an episomal mammalian expression vector that uses thecytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
pCEP4是pCEP4/CAT的對照質(zhì)粒，插入一個基因CAT.

質(zhì)粒圖譜

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網(wǎng)址：www.biovector.net