Laconic/pcDNA3.1(-)
啟動子: CMV,T7，SV40
復制子: pUC ori,f1 ori,SV40 ori
終止子: SV40 poly(A) signal,bGH poly(A) signal
質粒分類: 哺乳細胞，乳酸生物傳感器
質粒大小: 7648bp
原核抗性: Amp
克隆菌株: DH5α
培養條件: 37℃，有氧 LB
表達宿主: 哺乳細胞
誘導方式: 無須誘導，瞬時表達
5'測序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'測序引物: BGH:TAGAAGGCACAGTCGAGG
質粒簡介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 μM to 10 mM and was not affected by glucose, pyruvate, acetate,

質粒圖譜

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