pAAV-DJ腺相關(guān)病毒包裝質(zhì)粒Rep-Cap BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

Adeno-associated virus (AAV) serotypes differ broadly in transduction efficacies and tissue tropisms. DNA family shuffling technology was used to create a complex library of hybrid capsids from eight different wild-type viruses (Figure 3). Stringent selection of AAV variants on human liver cells and with human anti-AAV antisera result in AAV-DJ (a clone named after the first two authors, see ref. 11), and AAV-DJ/8 (a heparin binding domain mutant of AAV-DJ). Recombinant AAV-DJ vectors mediate superior in vitro transduction efficacies in comparison with any other wild type serotypes. Transduction on cell types from different species and tissues, including primary human hepatocytes, melanoma cells, and embryonic stem cells, showed that AAV-DJ vectors were not only superior to all HBD-negative wild-type viruses (up to 100,000-fold better than AAV-8 or AAV-9), but also substantially better than AAV-2. (See Table 2 for detail). The heparin binding domain (HBD) plays important role for in vivo viral infection as demonstrated by comparing AAV-DJ to the DJ/8 mutant: HBD deletion alleviated the liver restriction and expanded transduction to all nonhepatic tissues, including the brain, identical to the transduction patterns of AAV-8 and AAV-9.

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