Gn1-7 cells下丘腦GnRH神經(jīng)細(xì)胞株 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
Description GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
Overview GT1-7 is an immortalized mature mouse hypothalamic GnRH neuronal cell line. Immortalized GnRH neurons were generated by introducing a transgene containing the promotor region of the GnRH gene coupled to the coding region of the SV40 T-antigen oncogene into transgenic mice1. The resulting anterior hypothalamic tumors were removed from one of the mice and the cells dissociated and cloned. GT1-7 is a clonal line of mature differentiated GnRH neurons that exhibit high levels of Gnrh1 mRNA and secrete GnRH in response to depolarization.
Alternate Names ? GT1-7 hypothalamic cell line
? GT1-7 neural cell line
Background Information Gonadotrophin-releasing hormone (GnRH) is a neuropeptide that is synthesized and released from GnRH neurons within the hypothalamus and is required for normal reproductive development and function. The scarcity and scattered distribution of GnRH neurons in the rostral hypothalamus make the biological study of the cells difficult.

References:
1. Mellon PL, et.al. (1990) Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 5(1): 1-10.
2. Liposits Z, et.al. (1991) Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone. Endocrinology 129(3): 1575-1583.
3. Wetsel WC, et.al. (1991) Metabolism of pro-luteinizing hormone-releasing hormone in immortalized hypothalamic neurons. Endocrinology 129(3): 1584-1595.
4. Wetsel WC, et.al. (1992) Intrinsic pulsatile secretory activity of immortalized luteinizing hormone-releasing hormone-secreting neurons Proc. Natl. Acad. Sci. USA 89(9): 4149-4153.
5. Whyte DB, et.al. (1995) A neuron-specific enhancer targets expression of the gonadotropin-releasing hormone gene to hypothalamic neurosecretory neurons. Mol. Endocrinol 9(4): 467-477.
Applications
Application GT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.
Key Applications ? Cell Culture
? Cell Based Assays
Biological Information
Cell Line Type ? Neural Lineage Cells

Comments Transformant: Simian virus 40 (SV40).
Misspelling: GTI-7; Occasionally.
Species of origin Mus musculus (Mouse)
Breed/subspecies: (C57BL/6J x BALB/cJ) F2.
Hierarchy Parent: (GT1)
Children:
(GT1-7-OX1)
Category Transformed cell line

【Supplier來源】BioVector NTCC Inc.
TEL：+86-010-53513060
【W(wǎng)ebsite網(wǎng)址】 http://www.biovector.net