pcDNA3-OVA

Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Gene/Insert:Ovalbumin (Gallus gallus)

Vector typeMammalian Expression

Selectable markersNeomycin (select with G418)

GROWTH IN BACTERIA

Bacterial Resistance(s)Ampicillin

Growth Temperature37°C

Growth Strain(s)DH5alpha

Copy numberUnknown

GENE/INSERT

Gene/Insert nameOvalbumin

SpeciesG. gallus (chicken)

PromoterCMV

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ cloning siteEcoRI (not destroyed)

3′ cloning siteXbaI (not destroyed)

5′ sequencing primerCMV Forward

3′ sequencing primerBGH-rev

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