pBT3-C Information
Function yeast Hybrid plasmid

Promoter: CYC1 promoter

Replicator: pUC ori, F1 ori, 2 mu ori

Terminator: CYC1 Terminator

Plasmid classification: yeast cell plasmids; yeast hybrid plasmids; two hybrid plasmids

Plasmid size: 7599bp

Plasmid label: C-Cub, C-LexA, VP16

Prokaryotic resistance: kanamycin Kan

Screening markers: LEU2

Cloned strains of Escherichia coli, DH5 A and other Escherichia coli

Culture conditions: 37 centigrade, LB, aerobic

Expression host: yeast

Culture conditions: 30 centigrade, YPD, aerobic

Note: high copy

pBT3-C Description

PBT3-C is a plasmid for yeast two hybrid experiment of membrane proteins.

The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
       Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein          or soluble protein
       Map domains or amino acids which are critical for an interaction
       Screen cDNA libraries using a membrane protein as a bait to find novel
       interacting proteins (requires purchase of a separate cDNA library from Dualsystems)

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