B82 cell line細胞株細胞系 BioVector NTCC質粒載體菌種細胞基因保藏中心
Cell Line Name: B82


Keywords: Mouse C3H/An sc areolar and adipose tissue
Cell Line Description: Derivative of clone 929 obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.
Species: Mouse
Tissue of Origin: adipose

CellType: Fibroblast

Growth Mode: Adherent
Karyotype: 2n = 51


Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Country: UK
References: Exp Cell Res 1966;41:190; J Cell Sci 1969;5:93

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