pGL4.29[luc2P/CRE/Hygro]哺乳動(dòng)物細(xì)胞報(bào)告質(zhì)粒
pGL4.29[luc2P/CRE/Hygro]哺乳動(dòng)物細(xì)胞螢火蟲熒光素酶報(bào)告質(zhì)粒載體，Amp抗性，hygro潮霉素細(xì)胞篩選標(biāo)記
啟動(dòng)子: SV40 復(fù)制子: pUC ori，SV40 ori 終止子: SV40 poly(A) signal 質(zhì)粒分類: 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 質(zhì)粒大小: 6084bp 原核抗性: Amp 篩選標(biāo)記: Hyg 克隆菌株: DH5α 培養(yǎng)條件: 37℃，有氧 LB 表達(dá)宿主: 哺乳細(xì)胞 誘導(dǎo)方式: 無須誘導(dǎo)，瞬時(shí)表達(dá) 5'測(cè)序引物: RVP3:CTAGCAAAATAGGCTGTCCC 3'測(cè)序引物: 根據(jù)序列設(shè)計(jì)引物 質(zhì)粒簡(jiǎn)介 The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance 質(zhì)粒圖譜
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