pGL4.13[luc2/SV40]哺乳動物細胞熒光素酶質(zhì)粒
pGL4.13[luc2/SV40]哺乳動物細胞熒光素酶報告質(zhì)粒載體，Luc2熒光報告基因，SV40 early增強子-啟動子，Amp抗性
啟動子: SV40 復制子: pUC ori 終止子: SV40 poly(A) signal 質(zhì)粒分類: 哺乳細胞，信號通路報告載體 質(zhì)粒大小: 4641bp 原核抗性: 氨芐青霉素Amp 克隆菌株: 大腸桿菌DH5α 培養(yǎng)條件: 37℃，有氧 LB 表達宿主: 哺乳細胞 誘導方式: 無須誘導，瞬時表達 5'測序引物: RVP3:CTAGCAAAATAGGCTGTCCC 3'測序引物: 根據(jù)序列設(shè)計引物 質(zhì)粒簡介 The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) gene, have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription. 質(zhì)粒圖譜
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