pMIR-Report Luciferase質粒
啟動子: CMV,SP6,T7,Lac 復制子: pUC ori，SV40 ori 終止子: SV40 poly(A) signal 質粒分類: 哺乳細胞，信號通路報告載體 質粒大小: 6470bp 原核抗性: Amp 篩選標記: Puro 克隆菌株: DH5α 培養條件: 37℃，有氧 LB 表達宿主: 哺乳細胞 誘導方式: 無須誘導，瞬時表達 5'測序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG 3'測序引物: M13F：TGTAAAACGACGGCCAGT 質粒簡介 The pMIR-REPORT? miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.
質粒圖譜
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
電話：+86-010-53513060
網址：www.biovector.net