pSecTag2 A哺乳動(dòng)物細(xì)胞分泌表達(dá)質(zhì)粒
啟動(dòng)子: CMV 復(fù)制子: pUC ori，F(xiàn)I ori 終止子: SV40 poly(A) signal,BGH poly(A) signal 質(zhì)粒大小: 5159bp 原核抗性: 氨芐青霉素Amp 篩選標(biāo)記: 博來霉素Zeo 克隆菌株: 大腸桿菌DH5α 培養(yǎng)條件: 37℃ 有氧，LB 表達(dá)宿主: 哺乳細(xì)胞 誘導(dǎo)方式: 無須誘導(dǎo)，瞬時(shí)表達(dá) 5'測序引物: T7:TAATACGACTCACTATAGGG 3'測序引物: BGH:TAGAAGGCACAGTCGAGG 質(zhì)粒簡介 pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest. 質(zhì)粒圖譜
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