pRevTRE哺乳動物細(xì)胞調(diào)控質(zhì)粒
啟動子: CMV 復(fù)制子: pUC ori 質(zhì)粒分類: 哺乳細(xì)胞，四環(huán)素調(diào)控系統(tǒng)載體 質(zhì)粒大小: 6487bp 原核抗性: Amp 篩選標(biāo)記: Hyg 克隆菌株: Stbl3 培養(yǎng)條件: 37℃，有氧 LB 表達(dá)宿主: 哺乳細(xì)胞 誘導(dǎo)方式: 四環(huán)素誘導(dǎo) 5'測序引物: 根據(jù)序列設(shè)計引物 3'測序引物: 根據(jù)序列設(shè)計引物 質(zhì)粒簡介 pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tetresponse element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Ψ+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously (2, 3). pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria. 質(zhì)粒圖譜
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