Super PiggyBac Transposase (PB200PA-1)哺乳動物細胞轉座質粒
啟動子: Polr2A promoter 復制子: pUC ori,f1 ori 終止子: SV40 poly(A) signal 質粒分類: 哺乳細胞轉座酶表達質粒 質粒大小: 8531bp 原核抗性: 氨芐青霉素Amp 克隆菌株: 大腸桿菌Stbl3 培養條件: 37℃，有氧，LB 表達宿主: 哺乳細胞 誘導方式: 無須誘導，瞬時表達 5'測序引物: M13R:CAGGAAACAGCTATGACC 3'測序引物: 根據序列設計引物 質粒簡介 Super PiggyBac Transposase (PB200PA-1)是一個哺乳細胞轉座質粒。The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells. 質粒圖譜
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