pCpGL-CMV/EF1?(positive control, CpG-free)?pCpGL is a vector designed for reporter studies on the effect of promoter CpG methylation. The vector is completely CpG-free and its backbone does not influence the activity of the luciferase reporter gene after in vitro treatment with CpG methylases like SssI, HhaI or HpaII.
The pCpGL plasmids contain the R6K gamma origin of replication and require special E.coli strains, like PIR1 (Invitrogen), GT115 (Invivogen). The plasmid also carries a ZEOCIN resistence gene.
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