pMMB66EH plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

P. stutzeri A1501-WT wild strain of P. stutzeri A1501
P. stutzeri A1501-pk18-d2hgdh' P. stutzeri A1501 strain harboring chromosomal integration with pk18-d2hgdh' plasmid; Kmr *
P. stutzeri A1501-pk18-etf' P. stutzeri A1501 strain harboring chromosomal integration with pk18-etf' plasmid; Kmr *
P. stutzeri A1501-Δd2hgdh P. stutzeri A1501 strain mutant obtained by exchanging the d2hgdh gene with the d2hgdh'
P. stutzeri A1501-Δetf P. stutzeri A1501 strain mutant obtained by exchanging the etf gene with the etf'
P. stutzeri A1501-Δd2hgdh-d2hgdh+ A1501-Δd2hgdh strain harboring the plasmid pBBR1MCS-5-d2hgdh; Gmr *
P. stutzeri A1501-Δetf-etf+ A1501-Δetf strain harboring the plasmid pBBR1MCS-5-etf; Gmr *
P. stutzeri A1501-Δd2hgdh-dmlA+ A1501-Δd2hgdh strain harboring the plasmid pBBR1MCS-5-dmlA; Gmr *
P. stutzeri A1501-pME6522-Pd2hgdh P. stutzeri A1501 strain harboring the transcriptional fusions plasmid pME6522-Pd2hgdh
BL-D2HGDH E. coli BL21(DE3) strain harboring the expression plasmid pET-d2hgdh
BL-ETF E. coli BL21(DE3) strain harboring the expression plasmid pET-etfAB
BL-SerA E. coli BL21(DE3) strain harboring the expression plasmid PET-serA
BL-DmlA E. coli BL21(DE3) strain harboring the expression plasmid PET-dmlA
E. coli K-12 MG1655-WT wild strain of E. coli MG1655
E. coli K-12 MG1655-ΔdmlA E. coli MG1655 strain mutant obtained by exchanging the dmlA gene with the Kana
E. coli K-12 MG1655-ΔdmlA-dmlA+ MG1655-ΔdmlA strain harboring the plasmid pMMB66EH-dmlA; Apr *
E. coli K-12 MG1655-ΔdmlA-d2hgdh+ MG1655-ΔdmlA strain harboring the plasmid pMMB66EH-d2hgdh; Apr *
Plasmid
pk18mobsacB Allelic exchange suicide vector mobilized by E. coli S17-1λpir; for selecting double crossover; KmrSucs *
pk18-d2hgdh' pK18mobsacB carrying d2hgdh'
pk18-etf' pK18mobsacB carrying etfAB'
pET28a(+) vector for protein expression, Kmr *
S-3
pET-dmlA pET28a(+) carrying dmlA gene
pBBR1MCS-5 broad host range cloning vector; Gmr *
pBBR-dmlA pBBR1MCS-5 carrying dmlA gene
pMMB66EH broad host range tacP expression vector; lacIq ; Apr *
pMM-d2hgdh pMMB66EH carrying d2hgdh gene
pMM-dmlA pMMB66EH carrying dmlA gene
pME6522 E. coli–Pseudomonas shuttle vector for transcriptional lacZ fusions and promoter probing, Tcr *
pME6522-Pd2hgdh A 199 bp fragment upstream translation initiation site of d2hgdh was ligated into the pME6522
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