Clostridium ljungdahlii strain菌株菌種 BioVector NTCC質粒載體菌種細胞基因保藏中心

Designation: PETC
Deposited Name: Clostridium ljungdahlii Tanner et al.
Product Description: Type Strain. Redeposit of ATCC 49587
Medium
ATCC? Medium 1754: PETC medium
Growth Conditions
Temperature: 37°C
Atmoshere: Anaerobic gas mixture, 80% H220%
CO2 or 80% N220%
CO2 or 80% N210%
CO210%
H2
Propagation Procedure
1. This culture must be propagated under strictly anaerobic, conditions (see below).
2. Just prior to inoculation, place the frozen vial at room temperature to thaw.
3. Under anaerobic conditions, aseptically transfer the entire contents of the vial to a tube of PETC broth
(5 to 6 mL).
4. Additional #1754 broth tubes may be inoculated by transferring 0.5 mL of the primary tube to each
additional broth tube. A slant and a prereduced
blood plate may be inoculated with 0.1 mL each of the
cell suspension. Also inoculate a nonselective
plate and tube of broth to test for the presence of
aerobic contaminates.
5. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate aerobic plate and broth tube at
37°C.
6. Within 3 to 4 days, growth should be evident by good turbidity in the broth, and flaky growth on the
agar slant. No growth should occur on the blood agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
Use of an anaerobic gas chamber, or
Placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in anaerobic chamber,
Loose screw