NCI-H358 cell line細胞株細胞系 BioVector NTCC質粒載體菌種細胞基因保藏中心
Organism Homo sapiens, human
Tissue lung/bronchiole; derived from metastatic site: alveolus
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease bronchioalveolar carcinoma; non-small cell lung cancer
Gender male
Applications
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
Derivation
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
Clinical Data
male
Genes Expressed
lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
Cellular Products
lung surfactant associated protein A (SP-A)
Tumorigenic Yes
Effects
Yes, the cells produce tumors in athymic nude mice
Comments
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
SP-B and SP-C RNA was not expressed.
They have a reported colony forming efficiency of 0.83% in soft agarose.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17
Population Doubling Time 38 hrs
【Supplier來源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website網址】 http://www.biovector.net